Abstract: The method of evaluating resistance to carrot black rot at seedling stage was established and the resistant varieties were screened. Identification of disease resistance to carrot black rot was performed by artificial spray inoculation at seedling stage of the carrot in the same field for two years, the evaluation standard of resistance types was established and clustering analysis of carrot varieties was evaluated according to relative resistance index (RRI) comparison. The evaluation standard of resistance was establish as: HR, RRI≥ 1.4; R, 1.1≤RRI＜1.4; MR, 0.5≤RRI＜1.1; S, 0.1≤RRI＜0.5; HS, RRI＜0.1. The tested carrot varieties including two varieties of high resistant type, three varieties of resistant type, three varieties of middle resistant type, one variety of middle resistance sensitive type and two varieties of high sensitive type. The method and the standard of resistance evaluation to carrot black rot in this study could be the basis for resistance breeding and species selection.