Acquisition and Sequence Analysis of MuLigⅠ Gene from Mulberry (Morus alba L.)

doi:10.11924/j.issn.1000-6850.casb14120166

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (8): 113-117.doi: 10.11924/j.issn.1000-6850.casb14120166

Special Issue: 生物技术；园艺

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Acquisition and Sequence Analysis of MuLigⅠ Gene from Mulberry (Morus alba L.)

Wang Taoni, Kong Weiqing, Yang Jinhong

(The Key Sericultural Laboratory of Shaanxi, Ankang University, Ankang Shaanxi 725000)

Received:2014-12-24 Revised:2015-01-06 Accepted:2015-01-07 Online:2015-04-07 Published:2015-04-07

Abstract

Abstract: For further study on the role of DNA ligase Ⅰ in the replication of viroid, the DNA ligase Ⅰ gene was obtained from MMDVd-infected mulberry leaf. The gene was amplified by degenerate primer designed in the conserved domain according to the homologous genes and RACE methods. The results showed that the mRNA sequence of MuLig Ⅰ was 2 730 bp in length. The open reading frame (ORF) was 2367 bp and encoding 788 amino acid residues. There were conserved DBD domain (173-351 aa), catalytic domain (405-771 aa), and mitochondrion and nuclear location signal (MLS and NLS) in MuLigⅠ. The MuLig Ⅰ gene in this study contained conserved structural domain and active site, and like the DNA ligase Ⅰ gene of tomato, might be involved in the replication of MMDVd.

Wang Taoni,Kong Weiqing and Yang Jinhong. Acquisition and Sequence Analysis of MuLigⅠ Gene from Mulberry (Morus alba L.)[J]. Chinese Agricultural Science Bulletin, 2015, 31(8): 113-117.

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Acquisition and Sequence Analysis of MuLigⅠ Gene from Mulberry (Morus alba L.)

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