Abstract: Kiwifruit bacterial canker is a devastating disease, which is difficult to be controlled and spreads rapidly. Therefore, it has severely hampered the development of kiwifruit industry. In order to find a way to control kiwifruit canker, 25 isolates of Bacillus spp. were obtained from the rhizosphere soil of kiwi plant, in which 4 isolates could inhibit the growth of Pseudomonas syringae pv. actinidiae and the inhibition zone diameter reached more than 5 mm. B2 was the optimal strain and preliminarily identified as Bacillus cereus by morphological characters of bacterial colonies and individuals, physiology-biochemistry properties and 16S rDNA sequence alignments. 1×10⁹ cfu/mL B2’s fermentation liquid was diluted 50, 100, 500, 1000 and 2000 times for pot experiment. By spray method, after 30 days the control effect reached 90.45%, 86.52% and 80.90%, 67.98% and 47.19% and 15.73%, respectively. After 60 days, with the emergence of new disease spot, old spots became larger than before, but compared with CK, spot increase rate slowed down significantly. Field experiment showed that the strain B2 was a potential biocontrol Bacillus against P. syringae pv. actinidiae, 50 times dilution of B2’s fermentation raw liquid by spraying or 100 times by smearing could effectively reduce the hazards of kiwifruit bacterial canker.