Abstract: Seedling produced by plant tissue culture is an important technological approach that could solve the edible roses seedling market shortage. In this approach, the key is to realize the high efficiency and high quality stems proliferation. For this, a crossed experiment of L₉(3⁴) was conducted on Dianhong edible rose to study the effects of different factors on stem segments proliferation and screen the best proliferation medium. The results showed that there were great differences in stem segments differentiation and proliferation of Dianhong edible rose among the treatments, and there were 4 main plant morphology types. Basal medium had a significant effect on rose’s proliferation coefficient (P＜0.05), but effect differences of 6-BA, NAA and GA₃ on proliferation coefficient were not significant (P＞0.05). The effects of 4 factors on proliferation coefficient according to their influencing differences were as follows: basal medium＞6-BA＞NAA＞GA₃. The best stem segments proliferation medium of Dianhong edible rose was WPM + 6- BA 1.0 mg/L + NAA 0.01 mg/L + GA₃ 0.5 mg/L+ sucrose 30 g/L, which had some excellent characteristics such as high formation plant rate, high plant, straight and neat, stout stem and large proliferation coefficient, etc. Therefore, the results of this study could be applied to industrial tissue culture seedling of Dianhong edible rose.