Abstract: To determine whether Se can inhibit the growth of Sclerotinia sclerotiorum which was isolated from rape or not, experiments of plate culture and liquid medium culture were used here. In the present study, the germination, growth, inhibition, lethal effect, and the leakage of protein and nucleic acid material in cellular of Sclerotinia sclerotiorum which was treated with different concentrations of exogenous Se were determined. As the results showed, the growth of Sclerotinia sclerotiorum was inhibited significantly (P＜0.05), and the EC₅₀ was 2.23 mg/L. Se only inhibited the growth of Sclerotinia sclerotiorum but not led it to death. Low concentration of Se (＜5 mg/L) had no effect on inhibiting the germination of sclerotia of Sclerotinia sclerotiorum. However, the germination of sclerotia was inhibited totally when the concentration of Se was higher than 50 mg/L. The leakage of protein (OD₂₈₀) and nucleic acid material (OD₂₆₀) in cellular of Sclerotinia sclerotiorum increased with the treatment of Se, which indicated that the permeability of cell membrane was enlarged significantly with the addition of Se in the media. In conclusion, exogenous Se can inhibit the growth of Sclerotinia sclerotiorum. The results of the present study provide a theoretical basis for the safe agricultural action and the use of the micro-element Se.