Abstract: In vitro culture of unfertilized ovary of cabbage (Brassica oleracea L.) is affected by many factors (genotypes, growth period, phytohormones and its concentration, temperature, NaClO concentration for sterilization and treated time). This study aims to analyze the induction rate under various conditions, screen out a technique that can effectively induce unfertilized ovary to be a plant in vitro through single-factor and multiple-factor analysis experiments, and understand the development of unfertilized cabbage ovary in vitro. The results showed that genotype RM had the best induction effect, and the explant was successfully induced when it was in early flowering stage. The suitable media component for callus induction was MS 0.5 mg/L 6-BA 2.0 mg/L 2,4-D 0.1 mg/L NAA, 28.57% was the highest induction rate of callus and gynogenic embryos. The best and most suitable media component for adventitious bud was MS 0.1 mg/LNAA 0.2 mg/L 6-BA 0.05 mg/L TDZ , the regeneration frequency was up to 57.12%, adventitious buds and embryos could root and develop into complete plants when they were transplanted in the media (1/2MS 0.1 mg/L NAA). The average bud points and bud induction rate were the highest at 33℃ for 24 h, which were 2.85 and 100%, respectively. Treated with NaClO of 2% concentration for 15 min, the average bud points and bud induction rate were the highest, which were 2.93 and 97%, respectively.