Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (36): 259-264.doi: 10.11924/j.issn.1000-6850.casb15080002

Special Issue: 畜牧兽医

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Detection of Swine Derived Ingredients in Animal Derived Foods

Chen Zhengfang1, Zeng Ping1, Yang Jutian1, Zang Rongxin1, Cao Xin2, Xu Hongwei2   

  1. (1College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030;2Scientific Experimental Center, Northwest University for Nationalities, Lanzhou 730030)
  • Received:2015-08-01 Revised:2015-09-18 Accepted:2015-09-25 Online:2015-12-30 Published:2015-12-30

Abstract: To establish a specific method detecting the swine derived ingredients in some animal derived foods, a real-time PCR was established according to the conserved sequences of swine mtDNA, specific primers were designed and the animal derived products of bovine, ovine and swine were amplified. The target genes were cloned and reaction system was optimized to improve detection sensitivity. Different samples were tested repeatedly to test the stability of the detection method. The results demonstrated that only swine derived ingredients in those animal derived foods were detected. And the detection sensitivity reached 1×10-6 ng/μL. The established method was excellent for identification of swine derivatives in animal derived foods as it was accurate, cheap, convenient and fast.

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