Abstract: In order to understand the difference of seedling quality and yield between tomato test-tube plantlet and seedlings, and ensure the application of tomato test-tube plantlet in practical production, a comparative test on the seedling growth and early productivity of tomato test-tube plantlet and seedlings was conducted. The results showed that, the difference of seedling growth and early productivity between the two kinds of tomato seedlings was significant. Compared to seedlings, the test-tube plantlet displayed faster growth with developed root system, more dry matter accumulation and better seedling quality such as root-shoot ratio, G value, seedling index and root vigor. The relative dry matter accumulation in test-tube plantlet was as follows: leaf＞shoot＞root, while that in seedlings was: leaf or shoot＞root. The relative dry matter accumulation of root in test-tube plantlet was much higher than that in seedlings. The test-tube plantlet showed decreased first flower node, advanced flowering, large number and ratio of fruitlet, higher early productivity and theory productivity. Therefore, the tomato test-tube plantlet would be very suitable in practical production. Since the transplant survival rate of test- tube plantlet was low, the relative skills, such as seedling adaption and transplant, needed to be further studied.