Abstract: Research progresses in bovine intracytoplasmic sperm injection were reviewed in this paper. Owed the credit to the innovation of technology, rates of blastocysts increase to 40.1% from 1.8% reported first time in1990. Here, it is given a general overview on the effects of piezo-electric actuator and something else technology, the impacts of pre-treatment of spermatozoa before ICSI, the promoting of various activation treatments after ICSI and the prospect of using ICSI on animal husbandry and transgenesis studies.