The Clone and Sequence Analysis of BiCDPK Conserved Sequence from Bothriochloa ischaemum

doi:10.11924/j.issn.1000-6850.casb16040171

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (24): 130-135.doi: 10.11924/j.issn.1000-6850.casb16040171

Special Issue: 生物技术；畜牧兽医

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The Clone and Sequence Analysis of BiCDPK Conserved Sequence from Bothriochloa ischaemum

Wang Yunqi¹, Fang Zhihong¹, Ren Binlin¹, Li Lianfen¹, Dong Kuanhu²

(¹Animal Husbandry and Veterinary Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030032;²College of Animal Science and Technology, Shanxi Agricultural University, Taigu Shanxi 030801)

Received:2016-04-27 Revised:2016-08-04 Accepted:2016-06-24 Online:2016-08-29 Published:2016-08-29

Abstract

Abstract: In order to study the structure and function of CDPK gene, BiCDPK was cloned from Bothriochloa ischaemum by using RT-PCR method, and analyzed by bioinformatics. The results showed that the length of cDNA for BiCDPK was 1048 bp, which contained a 387 bp complete open reading frame encoding 128 amino acids. The corresponding protein was mildly acidic, stable and hydrophilic, with random coil and α-helix as main components in secondary structure. Subcellular localization indicated that the protein was located in cytoplasmic without transmembrane region and signal peptide. The sequence of protein included one serine phosphorylation sites and three threonine phosphorylation sites. Further phylogenic analysis displayed that BiCDPK in Zea mays, Triticum aestivum and Oryza sativa shared 97%, 86% and 86% identity. It laid a foundation to further study the BiCDPK function in Bothriochloa ischaemum.

Wang Yunqi,Fang Zhihong,Ren Binlin,Li Lianfen and Dong Kuanhu. The Clone and Sequence Analysis of BiCDPK Conserved Sequence from Bothriochloa ischaemum[J]. Chinese Agricultural Science Bulletin, 2016, 32(24): 130-135.

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The Clone and Sequence Analysis of BiCDPK Conserved Sequence from Bothriochloa ischaemum

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