Abstract: We carried out a research on NPK fertilizer response of the super high- yield rice variety ‘Liangyou616’grown in vegetable field with‘3414’the optimum regression design. The results indicated that balanced fertilization could effectively improve the agronomic characters of‘Liangyou616’in high soil fertility, and increase efficient panicle, grain per panicle and 1000-grain weight. The average yield-increasing effect of N, P and K applications was 13.1%, 2.9% and 13.4%, respectively, the yield-increase effect of N and K was higher than that of P. The uptake of N and P nutrients was mainly stored in rice, which was 63.8% and 73.6% of total uptake of N and P respectively, while 89.0% of potassium was stored in the straw. The utilization ratio of N, P and K fertilization in season was 34.7% , 24.7% and 30.2% , respectively. The economic application rates were N 71 kg/hm2, P2O5 34 kg/hm2 and K2O 101 kg/hm2, the optimum ratio of the three elements was 1:0.48:1.42 under the test condition. The results provided a scientific basis for adequate fertilization on‘Liangyou616’as post-vegetable rice.