Abstract: Twelve antagonistic strains, which inhibit the pathogen of kiwifruit cancer disease seriously, were screened out from rhizosphere soil. Activity of biologic inhibition was enhanced obviously, after purified by single-cell isolation. Especially, B17-4、 B56-1 and B56-3 were obvious. The result of field trial showed that control effect could get to 88%, by getting rid of infected tissue with smearing, when the filtration of B56-3 fermentation was dilute 500 times.