Abstract: The objectives were to reveal the effect of agronomic characters on cold tolerance of hybrid rice, analyze the correlation among main agronomic characters, and provide basis for breeding. The agronomic characters of 16 rice combinations including the whole growth period, plant height, effective spike, spike length, spike grain number, setting rate, yield and cold tolerance were analyzed by using the correlation analysis and path analysis. The results of correlation analysis showed that the real correlation coefficient of the whole growth period and plant height with cold tolerance reached significant level, and the whole growth period was the largest influencing factor. The results of path analysis showed that the whole growth period and plant height had a direct effect on the cold tolerance of hybrid rice. The correlation analysis and path analysis results consistently showed that the combination of longer whole growth period and higher plant height had stronger cold tolerance.