Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (29): 4-10.doi: 10.11924/j.issn.1000-6850.casb16060138

Special Issue: 生物技术 畜牧兽医

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Expression Comparison of Leptin Gene and Leptin Receptor Gene in Huai Pig and Duroc Pig

  

  • Received:2016-06-27 Revised:2016-08-13 Accepted:2016-08-16 Online:2016-10-12 Published:2016-10-12

Abstract: The study aims to find the differential expression of leptin gene (LEP) and leptin receptor gene (LEPR) in five adipose tissues of 7-month-old and 12-month-old Huai pig and Duroc pig. LEP gene and LEPR gene were amplified by fluorescence quantitative PCR, then relative expression was calculated by transferring CT value into 2- △ △ CT. Results showed that the expression of LEP was the highest in lower subcutaneous fat, followed by upper subcutaneous fat, abdominal subcutaneous fat and leaf fat, and was the lowest in coronary fat. Expression of LEP in adipose tissues of 7-month-old Huai pig had significant difference compared with that of 12-month-old Huai pig and 7-month-old Duroc pig (P<0.05). Compared with 7- month-old Duroc pig, expression of LEP gene in adipose tissues of 12-month-old Huai pig was extremely and significantly different (P<0.01). Compared with 12-month-old Huai pig, only the expression of LEPR gene in coronary fat of 7-month-old Huai pig was significantly different (P<0.05).

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