Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (20): 50-54.doi: 10.11924/j.issn.1000-6850.casb16070095

Special Issue: 园艺

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Purity Identification of Chinese Cabbage Hybrid‘Fuchun No.1’by SRAP Markers

  

  • Received:2016-07-18 Revised:2016-09-18 Accepted:2016-09-21 Online:2017-07-18 Published:2017-07-18

Abstract: ‘Fuchun No.1’is a new late bolting spring Chinese cabbage cultivar breeded by Fuzhou Institute of Vegetable Science. In order to identify the purity of‘Fuchun No.1’, 80 pairs of SRAP primers were used to amplify the DNA templates of‘Fuchun No.1’and its parental pooling. The pairs of primers which contained bands of parental complementary characteristics were screened out and used to identify the purity of‘Fuchun No.1’seedlings cultivated in field. In the study, the pairs of primer M2+E4 which contained bands of parental complementary characteristics were screened out and used to distinguish parents and hybrids effectively. The pairs of SRAP primer were adopted to identify the purity of 102‘Fuchun No.1’seedlings and the seed purity was 94.12% . The identification of SRAP markers is consistent with field survey results. SRAP molecular markers can be used to identify the purity of‘Fuchun No.1’.