Abstract: The aim is to explore the property and function of CGN in dairy cows. The bioinformatics was used to predict the amino acid sequence, secondary structure, tertiary structure and biological function of CGN, using online database biological database. Sequence analysis indicated that CGN was hydrophilic untransmembrane protein, and its cleavage position of the signal peptide was located between the 20th and 21st site of amino acid. The secondary structure of CGN was primarily composed of alpha helix and random coil, and the biological effects of CGN were performed in the plasma membrane. Biological function prediction indicated that the function of CGN might be involved in the biological process of hormone, growth factor, stress response and immune response. Phylogenetics analysis showed that the CGN of dairy cows was close to that of Bison, Bos mutus and Bubalus bubalis in the phylogenetic tree, which showed highly homology. This study provides a theoretical basis for further research on CGN and disease-resistant breeding of dairy cows.