Abstract: Mastering the evolution of the Autumn Low Temperature (ALT) in Guangxi can provide decision-making basis for late rice production. [Method]Based on the daily temperature data from 88 meteorological stations in Guangxi, we calculated the yearly beginning date and the total days of ALT, and analyzed the spatio-temporal variation characteristics of ALT in Guangxi by using linear tendency calculation, stage analysis, Morlet wavelet analysis, Mann-Kendall mutation test and correlation analysis. [Result] [Conclusion]The results showed that: (1) the beginning date of ALT tended to be delayed in most areas of Guangxi in recent 45 years, with a greater changing rate in the southern Guangxi than that in the northern; and the yearly ALT days tended to decrease in most areas of Guangxi, with a greater decreasing rate in the southern Guangxi than that in the northern; (2) ALT in Guangxi presented significant periodicity and mutation characteristics, whose mutation occurred in 1998; (3) the beginning date and yearly days of ALT existed quasi-periods of 4 and 8 years, separately; (4) ALT obviously responded to the climate warming. and the beginning date and yearly days of ALT had a significant positive and negative correlation with the mean temperature from September to October, respectively.