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Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (15): 71-75.doi: 10.11924/j.issn.1000-6850.casb16110139

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The Occurrence and Control of Vitrification Shoots of Populous suaveolens in Vitro Regenerative Culture

Zhao Xinwen, Liang Dejun   

  1. (Research Institute of Poplar of Liaoning Province, Gaizhou Liaoning 115200)
  • Received:2016-11-28 Revised:2017-05-05 Accepted:2017-04-19 Online:2017-05-26 Published:2017-05-26

Abstract: The vitrification of populous suaveolens was found during tissue culture. The influence factors on the reversion of vitrification were studied using orthogonal experimental design, include culture illumination, concentration of sucrose and agar. The results showed that concentration of sucrose have significant influence on the the reversion of vitrification, while the concentration of agar and the culture illumination have little. The rate of reversion can reach the highest 100% in the medium that added 9g/L sucrose, 20g/L agar, and with the culture illumination 3000lx. As a result, it is concluded that the medium adding higher concentration of sucrose would have great effect on the reversion of populous suaveolens.

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