Abstract: We aim to explore the regulation of mir-1298-5p in skin hair follicles which were at different development stages, and the potential relationship between mir-1298-5p and its target gene, so as to provide theoretical data for skin hair follicles development. Skin hair follicles from Liaoning cashmere goat were used as materials. Through miRNA isolation and expression, target gene prediction and screen, and construction of 3''UTR expression vector, the target gene of mir- 1298- 5p was predicted, sequencing and knocking down mutation of the target site were conducted. Results showed that 3''UTR of TGF-βR1 presented a binding site of mir- 1298- 5p, and mir- 1298- 5p and TGF- βR1 presented differential expression during different development stages of skin hair follicles. So mir- 1298- 5p might play an important role in periodic development of skin hair follicles. In addition, 3''UTR expression vector of TGF- βR1 was successfully constructed, laying a foundation for the construction of over expression transfection system and functional verification of target gene.