Abstract: The regenerated plant of cabbage was obtained by cultivating its unfertilized ovaryin vitro , effective ploidy identification of regenerated plant would lay a solid foundation for its further application in breeding and selecting excellent varieties of cabbage. In this study, three different-genotypes of cabbage (PMQM, QMF, RMQM) were chosen to produce new regenerated plants, improve the culture system of unfertilized ovary of cabbage in vitro , and identify the ploidy of regenerated plant by using morphological identification, chromosome counting in root-tip cells, and DNA flow cytometry. The results showed that the differentiation rates of callus of three genotypes cultivated in differentiation medium contained 0.4 mg/L ZT were significantly higher than that in the medium with 1.0 mg/L 6-BA, the RMQM genotype demonstrated the best differentiation efficiency. Finally, the medium MS 0.4 mg/L ZT 2.0 mg/L 2,4-D 0.1 mg/L NAA was identified as the most suitable medium for the differentiation of adventitious buds from callus. And through the three identification methods, the ploidy of regenerated plants was clarified as 3.4% haploid, 49.8% diploid, 15.9% tetraploid, and 35.3% chimera.