Distribution and Differential Expression of Apol8 in Mouse Normal Hatched Blastocysts and Dormant Embryos Before and After Cryopreservation

doi:10.11924/j.issn.1000-6850.casb17030120

Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (21): 149-153.doi: 10.11924/j.issn.1000-6850.casb17030120

Special Issue: 生物技术；畜牧兽医

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Distribution and Differential Expression of Apol8 in Mouse Normal Hatched Blastocysts and Dormant Embryos Before and After Cryopreservation

Wang Lihong, Guo Yong, Zhai Chundong, Lu Wenjin, Liu Di, Yang Jian, Wang Xiangguo,Sheng Xihui, Qi Xiaolong, Ni Hemin

(Animal Breeding and Assisted Reproductive Laboratory, Beijing University of Agriculture, Beijing 102206)

Received:2017-03-20 Revised:2017-07-10 Accepted:2017-05-01 Online:2017-07-27 Published:2017-07-27

Abstract

Abstract: This study aimed to investigate the distribution and differential expression of Apol8 protein in both mouse normal hatched blastocysts and dormant embryos before and after cryopreservation. The normal hatched blastocysts were obtained by superovulation, and the dormant embryos were collected from mice with delayed implantation. Confocal microscopy and western blot were applied to detect the distribution and differential expression of Apol8 among different groups. The results showed that Apol8, as an antifreeze protein, was expressed in mouse normal hatched blastocysts and dormant embryos before and after cryopreservation. The expression of Apol8 in normal hatched blastocysts after cryopreservation was 1.2 times that before cryopreservation (P＜0.01). The expression of Apol8 in dormant embryo after cryopreservation was 2.0 times that before cryopreservation (P＜0.01). The expression of Apol8 in dormant embryo after cryopreservation was 1.25 times that in normal hatched blastocyst after cryopreservation (P＜0.01). The expression level of Apol8 in dormant embryo was significantly higher than that of normal hatched blastocysts. Apol8 has the potential to be used as a novel mammalian-derived antifreeze protein.

CLC Number:

S814.8

Wang Lihong,Guo Yong,Zhai Chundong,Lu Wenjin,Liu Di,Yang Jian,Wang Xiangguo,Sheng Xihui,Qi Xiaolong and Ni Hemin. Distribution and Differential Expression of Apol8 in Mouse Normal Hatched Blastocysts and Dormant Embryos Before and After Cryopreservation[J]. Chinese Agricultural Science Bulletin, 2017, 33(21): 149-153.

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Distribution and Differential Expression of Apol8 in Mouse Normal Hatched Blastocysts and Dormant Embryos Before and After Cryopreservation

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