Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2019, Vol. 35 ›› Issue (3): 47-50.doi: 10.11924/j.issn.1000-6850.casb17100021

Special Issue: 生物技术 畜牧兽医

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In Vitro Culture of Fibroblast Cells from Simmental Ear Tissue

  

  • Received:2017-10-10 Revised:2017-11-13 Accepted:2017-12-21 Online:2019-01-28 Published:2019-01-28

Abstract: The establishment of donor animal cells in vitro culture model is necessary for animal cloning and production. In order to prepare bovine somatic cell nuclear transfer, the digestion and purification after tissue block culture method were used in vitro culture fibroblast cells in this experiment. The results showed that the fibroblast cell morphology was good, the passaged cell grew well with a high survival rate and good adhesion, and the proliferation of ear fibroblast cells accords with the general rule of cell growth in vitro using the digestion and purification after tissue block culture method. Therefore, tissue culture after digestion of purified bovine ear fibroblast cells is a simple and effective method, which can be used as a method for bovine somatic cell nuclear transfer preparation.