Abstract: Thirty hybrid combinations and their parents from 3 male sterile lines crossed to 10 restorer lines according NCII mode were employed to study the combining ability and heterosis for rice quality, and the results showed that: (i) Most rice quality traits of hybrid combinations showed the value between their parents, and the rice quality traits had no significant heterosis except the grain weight showed the over-high-parent heterosis, grain chalkiness had positive average heterosis. (ii) Male sterile lines and restorer lines had a great effect on the hybrid rice quality. For example, the 3 traits of grain length, grain width and amylose content, heavily depended on the male sterile lines, as for the quality traits of head rice rate, grain weight, chalky grain rate, grain chalkiness, alkali spreading value, et al, the impact of restorer lines was much higher than male sterile lines. (iii) Parents of guangzhan 63-4S, yangdao 6, shuhui 527 and zhenhui 084, have good GCA and SCA for quality traits and can be widely used for breeding new hybrid combinations with good quality. Parents of teqing and yanghui 559, showed the low GCA and SCA for quality traits and had no significant merits on rice hybrid quality breeding.