Chemical Components and Relevant Enzyme Activity of Lonicera japonica at Different Harvest Stages

doi:10.11924/j.issn.1000-6850.casb18030024

Abstract

Abstract: To further explore the dynamic change rule of active components in Lonicera japonica, we measured the contents of total phenol, total flavonoids, chlorogenic acid and the enzyme activities of phenylalanine ammonia lyase (PAL), cinnamic acid 4- hydroxylase (C4H), polyphenol oxidase (PPO), peroxidase (POD), superoxide dismutase (SOD) in L. japonica at different growth stages. And the correlation analysis was conducted. From bud stage to golden-flower stage, the contents of total phenol and flavonoids in L. japonica showed a trend of gradual decline, and the peak value was 4.42, 13.29 mg/g at bud stage respectively; chlorogenic acid reached the maximum at green bud stage, 33.37 mg/g. The contents of total phenol and flavonoids in leaves showed a fluctuation trend. Chlorogenic acid gradually declined, and the content was similar with that in flowers. The activity of C4H, PPO and POD had positive correlation with the content of active components. The active components in flowers and leaves are similar, so we can further develop the use value of leaves.

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[4] 王辉,田呈瑞,马守磊,等.绿原酸的研究进展[J].食品工业科学,2009,30(5):341-345．
[5] 兰小艳,张学俊,龚桂珍.杜仲叶中绿原酸的研究进展[J].中国农学通报,2009,25(21):60-62．
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