Abstract: The paper aims to uncover the characteristics of N, P and K uptake in different organs in wolfberry tree, and propose the reasonable application rates of chemical fertilizers. As the object of seven-years old tree and four-years young tree of wolfberries ‘Ningqi No.1’, the field experiments were conducted to study on the dynamics of N, P and K contents in fruit, branch and leaf, and investigate the dynamics of dry/fresh fruits yield and N, P and K uptake during the annual growth period in wolfberry tree, and provide the recommended rates of nitrogen, phosphorus and potassium fertilizers. The results indicated that nutrients contents in fruit, branch, and leaf in wolfberry tree were N>K>P. Total N and total K contents in branch were changed by double peak of “M” type, and total N contents in leaf were described by inverted “V” type. Total N and total K contents in different organs were in large variation, however, that of total P contents were little changed. Dynamics of dry/fresh fruits yield and N, P and K uptake in wolfberry were changed by the parabolic curves during summer and autumn fruit period, respectively; and the peak periods of nutrients accumulation in fruit were happened in the middle July and October, respectively. Relationships of yield and N, P2O5, K2O application rates were obeyed for quadratic curves, the recommended application rates of chemical fertilizers were N of 431-510 kg/hm2, P2O5 of 239-282 kg/hm2, and K2O of 362-379 kg/hm2 in young wolfberry tree. Therefore, N and K are the important nutrient elements in the different organs in wolfberry tree during the growth period, so as supplemental enough nutrients supplying of tree body by topdressing in the peak of nutrients demand.