Abstract: To clarify the potential of Glomus versiforme in alleviating continuous cropping obstacles, cucumber is selected as the experimental material. The seeds were inoculated with G. versiforme, and corresponding control was designed respectively. When the seedlings were 40 days old, they were transplanted to continuous cropping soil and sterilized soil, the effects of G. versiforme on growth, nitrogen and phosphorus uptake, and microorganism population in rhizosphere of cucumber plant were investigated. The results indicated that the effects of G. versiforme on shoot and root growth varied with soil condition. On the 55th day of emergence, shoot biomass of seedlings inoculated with G. versiforme in continuous cropping soil is 1.81 times that of its corresponding control, and the difference in the shoot biomass between mycorrhizal plant in continuous cropping soil and mycorrhizal seedlings in sterilized soil was not significant. Meanwhile, root biomass of seedlings inoculated with G. versiforme grown in sterilized soil were 1.22 times and 2.11 times that of mycorrhizal seedlings and nonmycorrhizal seedlings grown in continuous cropping soil, respectively. The significant shoot biomass of seedlings in continuous cropping soil is related to nitrogen improvement in leaves, and phosphorus concentration in root was enhanced by G. versiforme in sterilized soil. Moreover, G. versiforme was beneficial to improve soil environment, namely the number of bacteria and fungi in soil was increased and reduced, respectively. Thus, sterilizing or transplanting mycorrhizal seedlings to continuous cropping soil is an effective measure to relieve the continuous obstacle in cucumber facility cultivation.