Abstract: [Objective]In the study, the walnut shell were selected as the basic growth media and mixtures of pine bark and sawdust at a certain ratio, for an aim to resolve the problem such as the high cost of growth media and the deficient of the traditional growth media of D. officinale. Meanwhile, the study screened the best transplanting time after moved the root seedlings from theSlaboratory to the Greenhouse to improve theStransplantation survival rate. [Methods]To explore the best time for acclimatization and the feasibility of walnut shell as basic growth media of D. officinale, the walnut shell were selected as the basic growth media and used the main factors, including the different addition ratio of mixtures of pine bark and sawdust and the acclimatization time, toSdesign the L9(3)4 orthogonal experiment. [Results]The results showed that the periods for acclimatization of the seedlings have aSsignificantSimpactSonStransplantation survival rate; In the different sole growth media, the transplantation survival rate was higher in sawdust media than in the walnut shell and Pine bark media; but the seedlings grew better in Pine bark media than in walnut shell and both of them grew significantly better than in sawdust media. ThroughSorthogonal design, the result showed that moved the root seedlings from theSlaboratory to the Greenhouse about 14days were the best acclimatizationStime, and the mixtures of walnut shell, Pine bark and sawdust media at the ratio of 60%, 30% and 10% demonstrated the best growth of the seedlings. [Conclusion]The study indicated that it is feasible to cultivate D. officinale with mixed substrate based on walnut shell. This study provided a new utilization approach of the abundant walnut shell resources in Dali as well as a scientificSbasis for growing D. officinale in a large scale.