Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2019, Vol. 35 ›› Issue (7): 134-138.doi: 10.11924/j.issn.1000-6850.casb18100041

Special Issue: 园艺

Previous Articles     Next Articles

Determination of betaine in red Sugarbeet by hydrophilic interaction liquid chromatography-tandem mass spectrometryZhou Qin<sup>1,2,3,4</sup>, Wu Yumei<sup>1,2,3,4</sup>

  

  • Received:2018-10-12 Revised:2018-11-01 Accepted:2018-11-09 Online:2019-03-04 Published:2019-03-04

Abstract: The study aims to establish a quantitative analysis method for detecting betaine in red sugarbeet. By using the modified QuEChERS method combined with liquid chromatography-tandem mass spectrometry, the sample was extracted with methanol through accelerated solvent extraction(ASE), and purified by solid phase extraction, detected by hydrophilic interaction liquid chromatography- tandem mass spectrometry under multiple reaction monitoring mode and quantified by the external standard method. The results showed that linearity range was 0.05-5 μg/mL in the test. When the adding level was 4-16 mg/g, the recovery was 87.7%- 95.7%, and RSD was between 3.6% and 8.4%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.01 and 0.04 mg/L, respectively. The method is easy, quick, highly sensitive, and suitable for determination of betaine in sugabeet.

CLC Number: