Abstract: Since the transgenic technology of pollen tube pathway method was formally established, it has been widely used in crop genetic improvement. Extensive research shows: compared with other Transgenic technologies, the transgenic technology of pollen tube pathway method has the advantages of transform seeds can be directly got, high transform frequency, easy and simple to handle, The properties are steady, material variety, etc. This paper introduced the principle of transgenic technique by pollen tube pathway. This method is used to channel plants DNA into rice and variation like plant form, output properties, Disease-resistance, rice quality and Physiological biochemistry, etc. It is a easy and effective rice molecular breeding.