Abstract: The study of Paper mulberry is about its primary culture, secondary culture and rooting culture. The axillary and top buds are used as explants in primary culture, They are treated by ban guang an suang ahead of ten min, so it can prevent brown change and educate out bud clster for main. The optimum medium was MS+BA1.0mg/L+NAA0.1mg/L. Axillary and single-bud cegments were multiplied in secondary culture . The optimum medium was MS+BA0.5mg/L. It gains cane suger to make shine lower and alleviate alss variety phenomenon secondary seedling can take root but specd is slow, The optimum medium was 1/2MS+IBA0.5mg/L.