Abstract: To construct the fingerprint of sugarbeet varieties, two pairs of SRAP primer combinations (EM4- ME8 and EM7-ME10) with good polymorphism and clear and easy identification were obtained by screening 183 pairs of SRAP primer combinations. The fingerprints of 17 registered varieties were constructed by using the two pairs of primer combinations. The results showed that the primer combination EM7- ME10 could identify 15 varieties and amplify 15 different bands; the primer combination EM4-ME8 could identify 14 varieties and amplify 14 different bands; the two primer combinations combined could identify all 17 varieties. The success of using the SRAP primer combination to construct the fingerprint of sugarbeet varieties provides a new solution for the rapid identification of the authenticity of sugarbeet varieties, and provides a new way for protecting the rights of farmers and variety breeding professionals.