Exogenous Spd: Effects on Germination and Resistance of Snap Bean Seeds Under Low Temperature Stress

doi:10.11924/j.issn.1000-6850.casb19030030

Abstract

Abstract: In order to study the effect of exogenous Spd on seed germination and tolerance of snap bean under low temperature. In this study, the snap bean cultivar ‘Renaya’ was used as experimental material. Three treatments, normothermia control (CK), 4℃ low temperature treatment (LT) and addition of 1mmol/L Spd at 4℃ (LT+Spd) were used to determined germination rate, germination index, seed vigor and physiological index. The results showed that germination rate, germination index and seed vigor increased by 95.69%, 136.10% and 217.12%, respectively, after adding 1mmol/L Spd at 4℃; the electrolyte leakage rate decreased, the activities of antioxidant enzymes SOD, POD and CAT increased, and the contents of H2O2, O2-, MDA, proline and soluble protein decreased. Therefore, the application of exogenous Spd can alleviate the damage caused by low temperature stress on snap bean seeds, improve the low temperature tolerance, thereupon then improving the germination rate, germination index and seed vigor of snap bean seeds.

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