Abstract: The paper aims to collect and utilize the resources of new Lily varieties, and to promote and popularize the cultivated area of Lily varieties as a kind of under-forest flower, In this study, 8 cultivars of Lilium cultivars (‘Caesars Palace’, ‘Black Out’, ‘Donato’, ‘Eyeliner’, ‘Manissa’, ‘Ceb Dazzle’, ‘Brindisi’ and ‘Bonsoir’) were introduced and planted in Chenzhou, and the ornamental characteristics and adaptability were observed and evaluated by a score sheet. Meanwhile, experiments were carried out for the rapid propagation of Lilium by tissue culture technology, which based on the highest scores. Firstly, the results showed that ‘Caesars Palace’, ‘Black Out’, ‘Donato’ were suitable for planting in Chenzhou, and their scores were 4.78, 4.33 and 4.67, respectively. Secondly, the experiments of tissue culture, using the bulb of ‘Caesars Palace’ as explants, were indicated that the medium and inner of bulb scales would promote induction of shoots easier. Thirdly, the optimal medium for plantlets proliferation was MS medium containing 1.0 mg/L 6-BA and 0.2 mg/L NAA, and for root induction and elongation, 1/2MS medium plus NAA at the concentration of 0.5 mg/L showed good effect. Additionally, the survival rate of transplantation was about 85% under the optimal transplantation matrix of V (yellow soil): V(peat soil): V(perlite)= 5:4:1.