Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2003, Vol. 19 ›› Issue (4): 7-7.

Special Issue: 生物技术 畜牧兽医

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Analysis on Marker on Bovine Twinning trait with PCR-RFLP of FSHR Gene

LEI Xue-qin, , CHEN Hong, , XU Ting-sheng , YUAN Zhi-fa, LEI Chu-zhao, SUN Wei-bin, HU Shen-rong   

  • Online:2003-08-25 Published:2003-08-25

Abstract: PCR-RFLP in FSHR gene 5’–flanking region with exon 1 digested by restriction endonuclease TaqⅠdemonstrated that the frequencies of allele A (0.5500) and genotype AA (0.5000) in twinning cows were higher than that of allele A (0.3500) and genotype AA (0.3000) in monovular cows for Qinchuan cattle, while The frequencies of allele B (0.5937) and genotype BB(0.6875) in twinning cows were higher than that of allele B (0.5416) and genotype BB (0.4167) in monovular cows for Holstein cattle. Key words: Bovine; Twinning; FSHR gene; PCR-RFLP