Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2003, Vol. 19 ›› Issue (5): 12-12.

Special Issue: 生物技术 畜牧兽医

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Amplification of Mitochondria DNA from Mouse Single Preimplantation Embryo

Li Jun-feng Qian Min Lu Cheng Zhang Jia-hua   

  • Online:2003-10-25 Published:2003-10-25

Abstract: We have cultivated these cells from mice by KOH/DTT, such as single ovum, 2-cell embryo,8-cell embryo, morula and blastocyst. Then 5′ D-loop of mtDNA of these cells, which have 342bp, have been amplification by PCR technology. As a result, after these cells have cultivated by KOH/DTT, density of DNA, which has been tested by the mass UV-spectrophotometer, is 10~10.75mg/mL and OD260/OD280 is 0.835~0.944. Act these DNA as template DNA respectively, clear target DNA fragment could be attained by only one PCR amplification.