Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (19): 121-126.doi: 10.11924/j.issn.1000-6850.casb20190600347

Special Issue: 生物技术 园艺

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Control of Alternaria alternata by Mixed Fermentation of Trichoderma pseudokoningii and Rhizopus nigrican

Cong Yunzhe1, Ma Qunfei1, Yang Wenhui3, Chen Kaoshan1,2()   

  1. 1School of Life Science, Shandong University, Qingdao Shandong 266000
    2Suzhou Institute of Shandong University, Suzhou Jiangsu 215123
    3Shandong Pufang Biological Technology Co., Ltd., Yantai Shandong 265200
  • Received:2019-06-27 Revised:2019-10-17 Online:2020-07-05 Published:2020-07-08
  • Contact: Chen Kaoshan E-mail:ksc313@126.com

Abstract:

The research aims at the biological control effect of Rhizopus nigrican and Trichoderma pseudokoningii mixed fermentation broth on Alternaria alternata, and the development of a new environmental-friendly biological control agent. We conducted morphological identification and ITS identification on two biocontrol microorganisms. The growth rate method was used to determine the antibacterial activity of the mixed fermentation broth. In the field experiment in Laiyang of Yantai, the orchards with severe disease were treated with multiple irrigations and spraying of the broth for one year, and the control effect was measured and the activities of defense-related enzymes such as SOD, POD and PAL in apple leaves were determined. The results showed that the mixed fermentation of R. nigrican and T. pseudokoningii significantly improved the antibacterial ability against the pathogenic microorganism A. alternata. The antibacterial activity in vitro showed that the mixed fermentation broth had 66.73% antibacterial effect against A. alternata, which was significantly higher than the single fermentation broth of R. nigrican (51.23%) and T. pseudokoningii (17.28%). The field experiment showed that compared with the control group, the incidence of apple A. alternata leaf spot disease after treatment with the mixed fermentation broth decreased significantly and the relative control effect reached 42.46%. The results of leaf defense enzyme assay showed that the activity of SOD, POD and PAL of apple leaves increased by more than 50% after the treatment with mixed fermentation broth. Therefore, the mixed fermentation broth of R. nigrican and T. pseudokoningii has significantly inhibitory effect on A. alternata, and could effectively control apple A. alternata leaf spot disease and improve its disease resistance. The study lays a foundation for the development of new biological control agents.

Key words: Rhizopus nigrican, Trichoderma pseudokoningii, mixed fermentation, antibacterial activity, Alternaria, field experiment

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