Psoralea corylifolia L. Extract: Effect on Biological Activity of Rice Sheath Blight

doi:10.11924/j.issn.1000-6850.casb20191100835

Abstract

Abstract:

The purpose is to investigate the activity of Psoralea corylifolia L. extract on rice sheath blight. We studied the inhibition effects of Psoralea corylifolia L. extract against rice sheath blight by the hypha growth inhibition test, asclerotia germination inhibition test, SEM technologies and potted plant test, and verified the results by field trials. The results showed that EC₅₀ value of the inhibition effect of Psoralea corylifolia L. extract on mycelium growth of rice sheath blight was 30.8159 mg/L, the inhibition rate to sclerotium germination was 43.33% at 810 mg/L. SEM observation showed that hypha arrangement and morphology changed significantly after being treated by Psoralea corylifolia L. extract for 5 days. The results of potted plant test showed that the preventive and control effect of 150 times diluted 0.2% microemulsion against rice sheath blight was 94% and 78.46%, respectively; the field trials showed that the control effect of 0.2% microemulsion was 72.22% at 90 g/hm² after dosing once at intillering stage, booting stage and full heading stage, respectively. Psoralea corylifolia L. extract can inhibit mycelial growth and sclerotic germination of rice sheath blight, destroy its morphological structure, its microemulsion has remarkable control efficiency on rice sheath blight.

Key words: rice sheath blight, Psoralea corylifolia L. extract, isobavachalcone, antifungal activity, control effect

CLC Number:

S482.2+92

Wei Chang, Xu Qingyun, Li Yuejuan, Wang Jing, Guan Lijie. Psoralea corylifolia L. Extract: Effect on Biological Activity of Rice Sheath Blight[J]. Chinese Agricultural Science Bulletin, 2020, 36(30): 120-126.

Figures/Tables 9

References 26

[1]	刘畅. 辽宁省水稻纹枯病菌主要生物学性状分析及防控技术研究[D]. 沈阳:沈阳农业大学, 2018.
[2]	王爱军, 郑爱萍. 水稻纹枯病发病特点及防治措施[J]. 中国稻米, 2018,24(03):124-126.
[3]	胡千, 吴秀俊. 绩溪县水稻纹枯病发生特点及防治意见[J]. 乡村科技, 2019(25):83-84.
[4]	陈吉祥, 王勋芳. 彝良县水稻纹枯病发生特点与防控技术[J]. 云南农业科技, 2017(06):25-27.
[5]	茅忠权, 汪国文, 李章达, 等. 7种药剂对水稻纹枯病的防治效果[J]. 浙江农业科学, 2019,60(01):95-96.
[6]	李洪志, 杨呈芹. 宝应县不同药剂对水稻纹枯病的防治效果研究[J]. 植物医生, 2019,32(01):55-57.
[7]	曹奎荣, 陈婕, 王晔青, 等. 水稻纹枯病防治适期和对口药剂试验[J/OL]. 浙江农业科学, 2019(11):1978-1980[2019-11-12]. https://doi.org/10.16178/j.issn.0528-9017.20191109.
[8]	余露. 苯甲·嘧菌酯能兼防水稻纹枯病和稻瘟病[J]. 农药市场信息, 2019(19):53.
[9]	Han X B, Zhao J, Cao J M, et al. Essential oil of Chrysanthemum indicum L.: potential biocontrol agent against plant pathogen Phytophthora nicotianae[J]. Environmental science and pollution research international, 2019,26(7):7013-7023. doi: 10.1007/s11356-019-04152-y URL pmid: 30648233
[10]	何海燕, 柴荣耀, 邱海萍, 等. 五个抗稻瘟病基因在浙江省水稻品种中的分布和抗性评价[J]. 浙江农业学报, 2019,31(06):922-929.
[11]	方丽, 刘海青, 宋凤鸣, 等. 农杆菌介导的黄瓜炭疽菌遗传转化[J]. 浙江大学学报:农业与生命科学版, 2006(04):360-366.
[12]	Zhang Y Z, Chen Q, Liu C H, et al. Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat.[J]. Toxins, 2019,11(11):628. doi: 10.3390/toxins11110628 URL
[13]	董辰韵, 常瑶瑶, 钟伟强. 两亲性大分子季铵盐对水稻纹枯病菌抑菌活性表征方法研究[J]. 广东农业科学, 2019,46(06):86-92.
[14]	Huang X P, Luo J, Song Y F, et al. Favorable Bioactivity of the SDHI Fungicide Benzovindiflupyr Against Sclerotinia sclerotiorum Mycelial Growth, Sclerotial Production, and Myceliogenic and Carpogenic Germination of Sclerotia[J]. Plant disease, 2019,103(7):1613-1620. URL pmid: 30998449
[15]	雷海燕. 8%井冈霉素AS防治水稻纹枯病田间药效试验[J]. 福建农业科技, 2016(05):1-2.
[16]	李晓娟. 黄芩中抗蕃茄晚疫病原菌有效成分的提取及其抑菌机理的研究[D]. 呼和浩特:内蒙古农业大学, 2015.
[17]	易永丰, 周洁尘. 浅谈植物源杀菌剂[J]. 林业与生态, 2018(10):31-32.
[18]	关丽杰. 补骨脂抑菌活性成分及其制剂研究[D]. 沈阳:沈阳农业大学, 2008
[19]	梁财, 陈颖, 李昌恒, 等. 山芝麻提取物对10种植物病原真菌的抑菌活性初探[J/OL].广西植物, http://kns.cnki.net/kcms/detail/45.1134.q.20190516.1606.004.html, 2019-07-02.
[20]	杨苏苏, 赵慧琳, 程慧丽, 等. 22种植物提取物对番茄病原菌生物活性的筛选[J]. 山西农业科学, 2018,46(03):441-444.
[21]	杜亚楠. 新农抗702对水稻纹枯病抑菌机理的研究[D]. 南昌:江西农业大学, 2013.
[22]	母红岩. 核盘菌菌核的菌丝型萌发与侵染特性研究[D]. 武汉:华中农业大学, 2017.
[23]	陈维连, 赵燕, 石红军, 等. 戊二醛等对生物样品的影响[J]. 上海铁道大学学报(医学辑), 1999(09):85-86.
[24]	胡春辉, 徐青, 孙璇, 等. 几种典型扫描电镜生物样本制备[J]. 湖北农业科学, 2016,55(20):5389-5392,5402.
[25]	李彪, 韦刚, 孙俊铭, 等. 30%三环·戊唑醇悬浮剂防治水稻纹枯病研究[J]. 安徽农学通报, 2019,25(16):100-101.
[26]	张伟, 关丽杰. 0.2%苯丙烯菌酮微乳剂的田间应用研究[J]. 农药, 2018,57(11):843.

处理区	水稻分蘖末期		水稻孕穗期（破口前5~7天）		水稻齐穗期
处理区	药剂名称	用药量/ (mL/hm²)	药剂名称	用药量/(mL/hm²)	药剂名称	用药量/ (mL/hm²)
1	0.2%苯丙烯菌酮微乳剂	67.5	0.2%苯丙烯菌酮微乳剂	67.5	0.2%苯丙烯菌酮微乳剂	67.5
2	0.2%苯丙烯菌酮微乳剂	75	0.2%苯丙烯菌酮微乳剂	75	0.2%苯丙烯菌酮微乳剂	75
3	0.2%苯丙烯菌酮微乳剂	90	0.2%苯丙烯菌酮微乳剂	90	0.2%苯丙烯菌酮微乳剂	90
4	12%井冈.蜡芽菌水剂	375	井冈.蜡芽菌	375	井冈.蜡芽菌	375
5	2%春雷霉素水剂	90	春雷霉素	90	春雷霉素	90
6	空白对照	-	空白对照	-	空白对照	-

处理区	水稻分蘖末期		水稻孕穗期（破口前5~7天）		水稻齐穗期
处理区	药剂名称	用药量/ (mL/hm²)	药剂名称	用药量/(mL/hm²)	药剂名称	用药量/ (mL/hm²)
1	0.2%苯丙烯菌酮微乳剂	67.5	0.2%苯丙烯菌酮微乳剂	67.5	0.2%苯丙烯菌酮微乳剂	67.5
2	0.2%苯丙烯菌酮微乳剂	75	0.2%苯丙烯菌酮微乳剂	75	0.2%苯丙烯菌酮微乳剂	75
3	0.2%苯丙烯菌酮微乳剂	90	0.2%苯丙烯菌酮微乳剂	90	0.2%苯丙烯菌酮微乳剂	90
4	12%井冈.蜡芽菌水剂	375	井冈.蜡芽菌	375	井冈.蜡芽菌	375
5	2%春雷霉素水剂	90	春雷霉素	90	春雷霉素	90
6	空白对照	-	空白对照	-	空白对照	-

浓度/(mg/L)	D±S	抑制率/%	毒力回归方程	r	EC₅₀±SE/(mg/L)
CK	7.02±0.20	-	Y=3.3260+1.1244X	0.9846	30.8159±3.9142
3.3	6.37±0.30	10.53
10	5.02±0.40	32.41
30	3.65±0.30	54.62
90	2.48±0.50	73.58
270	2.00±0.40	81.36

浓度/(mg/L)	D±S	抑制率/%	毒力回归方程	r	EC₅₀±SE/(mg/L)
CK	7.02±0.20	-	Y=3.3260+1.1244X	0.9846	30.8159±3.9142
3.3	6.37±0.30	10.53
10	5.02±0.40	32.41
30	3.65±0.30	54.62
90	2.48±0.50	73.58
270	2.00±0.40	81.36

药剂	稀释倍数	病指/%	防效/%
CK	-	72.18	-
0.2%苯丙烯菌酮微乳剂	150	4.33	94.00a
	200	7.58	89.50ab
	250	15.05	79.15c
20%井冈霉素颗粒剂	500	4.90	93.21a
43%戊唑醇悬浮剂	1000	10.17	85.91b
25%嘧菌酯悬浮剂	1500	5.95	91.76ab