Abstract: Using Vicia faba as donor materials, the effects of seed–treatment on germination, supplementing AC, Vc and PVP to induction media on explant–browning and adding MET, banana, potato and coconut water to propagating media on propagating coefficient and bud–vitrifiation were investigated. The results showed that the frequency of germination were able to be increased by keeping seed vessel, culturing seeds with radicle up and pre-treating seeds in 5.0mg/L GA3 solution for 12h. The frequency of explant-browning was reduced by adding AC 0.5mg/L to induction media and the vigor of plantlets was promoted by the AC-adding. The propagating coefficient could be improved and the frequency of bud-vitrifration increased by adding 150mg/L coconut water.