Abstract: In order to accelerate development of honeysuckle industry, increase the number of honeysuckle young seedling and protect the ecological environment, the lateral bud of honeysuckle was developed. PLB was obtained through culture of stem with bud of wild honeysuckle on MS with BA0.1mg/L and NAA0.1mg/L. The best combination of hormone for proliferation of honeysuckle was MS with BA0.05mg/L and NAA0.1mg/L. 6-BA played a key role in the lateral bud formation and differentiation.