Abstract: Balance fertilization is required to accommodate plants with enough nutrients according to different soil and different crops. Their might be too many fertilizer prescriptions based on balance fertilization because of the difference of nutrients in different soil, and this is very difficult to put in practice. So it is urgent to seek a scientific and simple method to guide the decision making of fertilizer prescriptions in practice. In our study, 171 soil samples collected from tobacco growing areas in Nanyang county of Henan province were analyzed by the method of hierarchical cluster. The result showed that they were classified into 15 clusters, and the following analysis of ANOVA showed the differences between clusters are significant. Comparing the Mean and Std. D of every cluster’s nutrients value to the critical of soil nutrients in tobacco growing area, we put up 15 suggestions of fertilization according to 15 clusters, and selected 5 clusters which had more than 10 samples to prove the suggestions. The result showed that the prescriptions of fertilization according to hierarchical cluster analysis and balance fertilization are effective.