Effects of Exogenous Addition of Benzoic Acid on Seed Germination and Seedling Growth of Perilla frutescens

doi:10.11924/j.issn.1000-6850.casb2023-0178

Abstract

Abstract:

To investigate the effects of exogenous benzoic acid stress on seed germination, seedling growth and antioxidant enzyme activity of perilla seeds, perilla seeds petri dish filter paper culture and indoor potting test were conducted by adding benzoic acid, and six gradients were set: 0(CK), 1, 1.5, 2, 2.5 and 3 mmol/L. The effects of different concentrations of benzoic acid stress on germination percentage, germination energy, germination index, viability index, perilla seedling growth and antioxidant enzyme activities were studied. The results showed that with the increase of benzoic acid concentration, the germination rate, germination potential, germination index and viability index of perilla seeds were generally promoted by low concentration and inhibited by high concentration. The root weight, radicle length, cotyl length and plant fresh weight of perilla seedlings showed a significant downward trend, and the activities of catalase (CAT) and peroxidase (POD) in seedling leaves showed a significant decreasing trend (P<0.05), the activity of superoxide dismutase (SOD) first increased and then decreased, and reached maximum at 1.5 mmol/L. The effect of exogenous addition of benzoic acid on the germination of perilla seeds is related to the concentration of benzoic acid (low concentration promotion and high concentration inhibition), which has an inhibitory effect on the growth of perilla seedlings, confirming the self-toxicity effect of benzoic acid on the growth of perilla.

Key words: Perilla frutescens, benzoic acid, seed germination, antioxidant enzyme, allelopathic chemicals

XUE Tongtong, XIE Mengmeng, SUN Hui, LI Chongwei. Effects of Exogenous Addition of Benzoic Acid on Seed Germination and Seedling Growth of Perilla frutescens[J]. Chinese Agricultural Science Bulletin, 2024, 40(7): 72-77.

Figures/Tables 6

References 32

[1]	梁旭, 王宪龄, 陈德壮, 等. 紫苏籽油的研究和应用现状[J]. 中医学报, 2022, 37(3):547-551
[2]	ZHANG Henghui, ZHANG Zhijun, HE Dongliang, et al. Optimization of enzymatic hydrolysis of perilla meal protein for hydrolysate with high hydrolysis degree and antioxidant activity[J]. Molecules, 2022, 27(3).
[3]	姚琳, 夏伟, 余丽丽, 等. 紫苏籽油的提取工艺及生物学应用研究进展[J]. 当代化工, 2022, 51(8):1943-1947.
[4]	PANDEY A, BHATT K C. Diversity distribution and collection of genetic resources of cultivated and weedy type in Perilla frutescens (L.) Britton var. frutescens and their uses in Indian Himalaya[J]. Genetic resources & crop evolution, 2008, 55(6):883-892.
[5]	LIAO B G, HAO Y J, LU J X, et al. Transcriptomic analysis of Perilla frutescens seed to insight into the biosynthesis and metabolic of unsaturated fatty acids[J]. BMC genomics, 2018, 19(1).
[6]	YU H, QIU J F, MA L J, et al. Phytochemical and phytopharmacological review of Perilla frutescens L. (Labiatae), a traditional edible-medicinal herb in China[J]. Food and chemical toxicology, 2017, 108:375-391. doi: 10.1016/j.fct.2016.11.023 URL
[7]	ZENG J N, LIU J Z, LU C G, et al. Intercropping with turmeric or ginger reduce the continuous cropping obstacles that affect Pogostemon cablin (Patchouli)[J]. Frontiers in microbiology, 2020,11.
[8]	赵晓萌. 连作对西洋参生长发育的影响及根际化感物质的研究[D]. 北京: 中国协和医科大学, 2009:8-12.
[9]	LI Z B, FU J F, ZHOU R J, et al. Effects of phenolic acids from ginseng rhizosphere on soil fungi structure, richness and diversity in consecutive monoculturing of ginseng[J]. Saudi journal of biological sciences, 2018, 25(8).
[10]	谢星光, 陈晏, 卜元卿, 等. 酚酸类物质的化感作用研究进展[J]. 生态学报, 2014, 34(22):6417-6428.
[11]	程佳, 王发啟, 李小双, 等. 酚酸类化感物质种类、提取、分离和检测研究进展[J]. 江苏农业科学, 2022, 50(6):8-15.
[12]	蔡淼. 外源苯甲酸对穿心莲生长及品质影响的研究[D]. 广州: 广东药科大学, 2020:27-35.
[13]	李培栋, 王兴祥, 李奕林, 等. 连作花生土壤中酚酸类物质的检测及其对花生的化感作用[J]. 生态学报, 2010, 30(8):2128-2134.
[14]	焦焕然, 周冰谦, 杨国红, 等. 酚酸类化感物质介导病原真菌协同致害药用植物的研究进展[J]. 山东农业科学, 2021, 53(9):141-147.
[15]	ZHOU X, WU F. Effects of amendments of ferulic acid on soil microbial communities in the rhizosphere of cucumber (Cucumis sativus L.) - Science Direct[J]. European journal of soil biology, 2012, 50(3):191-197. doi: 10.1016/j.ejsobi.2012.03.001 URL
[16]	CUI J J, ZHANG E H, ZHANG X H, et al. Effects of 2,4-di-tert-butylphenol at different concentrations on soil functionality and microbial community structure in the Lanzhou lily rhizosphere[J]. Applied soil ecology, 2022,172.
[17]	耿贵, 杨瑞瑞, 於丽华, 等. 作物连作障碍研究进展[J]. 中国农学通报, 2019, 35(10):36-42. doi: 10.11924/j.issn.1000-6850.casb18110045
[18]	张治安, 张美善, 蔚荣海. 植物胜生理学实验指导[M]. 北京: 中国农业技术出版社, 2004:132-137.
[19]	KIM M J, CIANI S, SCHACHTMAN D P. A peroxidase contributes to ROS production during Arabidopsis root response to potassium deficiency[J]. Molecular plant, 2010, 3(2):420-427. doi: 10.1093/mp/ssp121 URL
[20]	MENG L F, ZHI X, LIU J S, et al. Extraction and GC-MS analysis of phenolic acids in rhizosphere soil of Pinellia ternate[J]. Journal of radiation research and applied sciences, 2022, 15(3):40. doi: 10.1016/j.jrras.2022.05.015 URL
[21]	韩春梅. 苯甲酸对白菜种子萌发及抗氧化酶活性的影响[J]. 种子, 2012, 31(10):114-115.
[22]	沙琦波. 信息技术支持下的深度教学设计——以“绿色开花植物的营养器官——根”为例[J]. 中学生物教学, 2021(34):55-57.
[23]	LI Z B, FU J F, ZHOU R J, et al. Effects of phenolic acids from ginseng rhizosphere on soil fungi structure, richness and diversity in consecutive monoculturing of ginseng[J]. Saudi journal of biological sciences, 2018, 25(8):1788-1794. doi: 10.1016/j.sjbs.2018.07.007 pmid: 30591801
[24]	MITTLER R, ZANDALINAS S I, FICHMAN Y, et al. Reactive oxygen species signalling in plant stress responses[J]. Molecular cell biology, 2022, 23(10):663-679.
[25]	BRATT A S, ROSRNWASSER S, MEYER A, et al. Organelle redox autonomy during environmental stress[J]. Plant, cell & environment, 2016, 39(9):1909-1919.
[26]	NIE W F, WANG M M, XIA X J, et al. Silencing of tomato RBOH1 and MPK2 abolishes brassinosteroid-induced H₂O₂ generation and stress tolerance[J]. Plant, cell & environment, 2013, 36(4):789-803.
[27]	赵可夫, 邹琦. 盐分和水分胁迫对盐生和非盐生植物细胞膜脂过氧化作用的效应[J]. 植物学报(英文版), 1993(7):519-525.
[28]	何雨沩. 低温胁迫下草莓NADPH氧化酶在ROS形成中的作用[D]. 雅安: 四川农业大学, 2015.
[29]	JAFARY K G, MORSHELOO M R, MUMIVAND H, et al. Natural diversity in phenolic components and antioxidant properties of oregano (Origanum vulgare L. ) accessions, grown under the same conditions[J]. Scientific reports, 2022, 12(1).
[30]	SARKER U, OBA S. Phenolic profiles and antioxidant activities in selected drought-tolerant leafy vegetable amaranth[J]. Scientific reports, 2020, 10(1):18287. doi: 10.1038/s41598-020-71727-y pmid: 33106544
[31]	KABTNI S, SDOUGA D, BETTAIB R I, et al. Influence of climate variation on phenolic composition and antioxidant capacity of Medicago minima populations[J]. Scientific reports, 2020, 10(1).
[32]	朱军, 徐小军, 张桂兰, 等. 不同化感物质对西瓜幼苗生长的影响[J]. 基因组学与应用生物学, 2018, 37(1):400-407.

浓度/(mmol/L)	发芽率/%	发芽势/%	发芽指数	活力指数
0	51.10±2.08a	17.78±3.38b	3.70±1.31bc	0.108±0.022ab
1	54.40±3.21a	37.78±1.86ab	5.43±1.09ab	0.122±0.014a
1.5	68.90±3.78a	52.22±2.16a	7.19±1.96a	0.130±0.020a
2	51.10±2.51a	34.44±1.45ab	4.98±0.29abc	0.062±0.002bc
2.5	50.00±2.00a	33.33±1.15ab	4.53±0.27bc	0.058±0.002c
3	48.90±2.51a	14.44±2.96b	3.13±0.87c	0.041±0.006c

浓度/(mmol/L)	发芽率/%	发芽势/%	发芽指数	活力指数
0	51.10±2.08a	17.78±3.38b	3.70±1.31bc	0.108±0.022ab
1	54.40±3.21a	37.78±1.86ab	5.43±1.09ab	0.122±0.014a
1.5	68.90±3.78a	52.22±2.16a	7.19±1.96a	0.130±0.020a
2	51.10±2.51a	34.44±1.45ab	4.98±0.29abc	0.062±0.002bc
2.5	50.00±2.00a	33.33±1.15ab	4.53±0.27bc	0.058±0.002c
3	48.90±2.51a	14.44±2.96b	3.13±0.87c	0.041±0.006c

浓度/(mmol/L)	胚根长/mm	胚轴长/mm	株鲜重/g
0	28.80±1.76a	15.90±1.74a	1.050±0.228abc
1	21.47±1.03b	12.10±1.01b	1.285±0.778a
1.5	19.13±1.34c	11.37±1.97b	1.025±0.393ab
2	9.27±0.50d	9.93±1.80bc	0.815±0.366bcd
2.5	7.83±0.32de	6.97±0.99c	0.550±0.160cd
3	5.8±0.46e	3.17±0.85d	0.295±0.064d

浓度/(mmol/L)	胚根长/mm	胚轴长/mm	株鲜重/g
0	28.80±1.76a	15.90±1.74a	1.050±0.228abc
1	21.47±1.03b	12.10±1.01b	1.285±0.778a
1.5	19.13±1.34c	11.37±1.97b	1.025±0.393ab
2	9.27±0.50d	9.93±1.80bc	0.815±0.366bcd
2.5	7.83±0.32de	6.97±0.99c	0.550±0.160cd
3	5.8±0.46e	3.17±0.85d	0.295±0.064d