Mutagenic Effect of 60Co-γ on Tissue Culture Seedlings of Paphiopedilum

doi:10.11924/j.issn.1000-6850.casb2021-0612

Abstract

Abstract:

The aims are to breed new varieties of Paphiopedilum, clarify the mutagenic effect of 60Co-γ on different developmental stages of Paphiopedilum and select appropriate radiation dose. Seeds, bottle seedlings (adventitious bud proliferation and adventitious root differentiation stage) and seedlings were used as materials which were treated under the radiation dose of 0 Gy, 5 Gy, 10 Gy, 20 Gy, 30 Gy and 40 Gy of ⁶⁰Co-γ. The growth and variation were observed and the physiological and biochemical indexes were measured. The results showed that the appropriate radiation dose for seed germination and adventitious root differentiation stage was 5 Gy, while the optimal radiation dose was 20 Gy for adventitious bud proliferation and seedling stage. 6.92 Gy was the semi-lethal dose for Paphiopedilum seeds. With the increase of radiation dose, the seed germination rate, adventitious bud growth rate, adventitious root differentiation rate, and seedling growth potential decreased and the variation increased. Simultaneously, the electrical conductivity, malondialdehyde content, soluble sugar content, soluble protein content, proline content and the activities of peroxidase and superoxide dismutase increased first and then decreased. The ideal radiation dose for Paphiopedilum was low at seed germination and adventitious root differentiation stage, and was relatively high at adventitious bud proliferation and seedling stage. The induced variation was obvious and the effect was stable with the appropriate radiation dose.

Key words: Paphiopedilum, radiation breeding, seed germination, differentiation, rooting

CLC Number:

S682.31

SUN Yin, HAO Jun, FANG Yifu, ZHANG Qian, JIANG Nannan. Mutagenic Effect of ⁶⁰Co-γ on Tissue Culture Seedlings of Paphiopedilum[J]. Chinese Agricultural Science Bulletin, 2022, 38(15): 45-52.

Figures/Tables 11

References 36

[1]	陈业, 石建明, 沈文华,等. 23种中国兜兰属植物亲缘关系的ISSR分析[J]. 西南大学学报:自然科学版, 2013, 35(6):15-21.
[2]	罗毅波, 贾建生, 王春玲. 初论中国兜兰属植物的保护策略及其潜在资源优势[J]. 生物多样性, 2003(6):491-498. doi: 10.17520/biods.2003059
[3]	陈庆文, 郭运青. 中国兜兰属植物研究现状综述[J]. 广西农业科学, 2010, 41(8):818-821.
[4]	曾宋君, 郭贝怡, 孔鑫平,等. 兜兰离体快繁技术研究进展[J]. 热带作物学报, 2020, 41(10):2080-2089.
[5]	张丰收, 王青. 植物辐射诱变育种的研究进展[J]. 河南师范大学学报:自然科学版, 2020, 48(6):39-49.
[6]	包建忠, 李风童, 孙叶,等. 辐射诱变技术在花卉育种中的研究与应用[J]. 南方农业, 2020, 14(14):183-186.
[7]	王晶, 刘录祥, 赵世荣, 等. ~(60)Co-γ射线对菊花组培苗的诱变效应[J]. 农业生物技术学报, 2006b(2):241-244.
[8]	刘丽强, 刘军丽, 张杰, 等. ~(60)Co-γ辐射对观赏海棠组培苗的诱变效应[J]. 中国农业科学, 2010a, 43(20):4255-4264.
[9]	姜长阳, 宁淑香, 杨文新,等. 愈伤组织辐射诱变选育玉兰新品系[J]. 园艺学报. 2002(5):473-476.
[10]	张慧琴, 谢鸣, 蒋桂华, 等. ~(60)Co-γ射线对草莓组培苗诱变效应[J]. 浙江大学学报:农业与生命科学版, 2007(2):180-183.
[11]	陈莹, 范旭丽, 高江云. 白旗兜兰种子非共生萌发与试管苗快速繁育[J]. 植物分类与资源学报, 2015, 37(5):611-615.
[12]	赵世杰, 刘华山, 董新纯. 植物生理学实验指导[M]. 北京: 中国农业科技出版社, 1998:68-72.
[13]	李合生. 植物生理生化实验原理和技术[M]. .北京: 高等教育出版社, 2000:195-197.
[14]	王瑞静, 王瑞文, 沈宝仙. ~(60)Coγ射线对杨树种子的辐射效应[J]. 核农学报, 2009, 23(5):762-765.
[15]	王丹, 任少雄, 苏军, 卫锋, 王熙, 2004.核技术在观赏植物诱变育种上的应用[J]. 核农学报, 18(6):443-447.
[16]	冯琪, 赵艺璇, 张晓飞, 等. ~(60)Co-γ辐射剂量对花葵种子萌发和幼苗生理特性的影响[J]. 新疆农业大学学报, 2019, 42(2):102-106.
[17]	杨再强, 王立新. 观赏植物辐射诱变育种研究进展[J]. 四川林业科技, 2006(3):19-23.
[18]	彭梦婕, 刘丹, 牛贺雨, 等. ~(60)Co-γ辐射对紫薇种子萌发、幼苗生长和生理的影响[J]. 安徽农业大学学报, 2020, 47(4):530-537.
[19]	袁帅, 董志晓, 赵煜晗, 等. ~(60)Co-γ辐射对扁穗雀麦种子萌发及其幼苗生长的影响[J]. 草业科学, 2021, 38(7):1319-1328.
[20]	赵艺璇, 孙桂芳, 杨建伟, 等. ~(60)Co-γ射线对2种园林植物种子萌发和幼苗生理特性的影响[J]. 西部林业科学, 2019, 48(1):119-124.
[21]	李瑜, 王萍, 耿兴敏, 等. ~(60)Co-γ辐射对桂花幼苗生长及生理指标的影响[J]. 西北农业学报, 2017, 26(1):61-69.
[22]	曾文丹, 严华兵, 曹升, 等. ~(60)Co-γ射线辐射对木薯组培苗的诱变效应[J]. 核农学报, 2019, 33(9):1667-1676.
[23]	陈淑英, 王玉英, 李叶芳,等. 洋桔梗CeremonyOrcmge品种辐射诱变育种[J]. 江苏农业科学, 2018, 46(10):134-137.
[24]	高健. 钴-60Gamma射线辐照中国水仙的诱变效应和机理研究[D]. 北京: 中国林业科学研究院, 2000.
[25]	陈秀兰, 孙叶, 包建忠,等. 君子兰辐射诱变育种研究初报[J]. 江苏农业科学, 2006(6):226-228.
[26]	张兴, 唐焕伟, 车代弟. 丰花月季~(60)Co-γ辐射育种研究及后代变异的初步分析[J]. 国土与自然资源研究, 2010(3):73-74.
[27]	汤章城. 逆境条件下植物脯氨酸的累积及其可能的意义[J]. 植物生理学通讯, 1984(1): 15-21.
[28]	杨婷. 膜脂过氧化对植物细胞的伤害[J]. 科技与创新, 2018(8): 61-62.
[29]	滕娟, 熊俊芬, 何忠俊, 等. ~(60)Co-γ辐射对三七幼苗生理特性的影响[J]. 云南农业大学学报:自然科学, 2015, 30(3):445-449.
[30]	ZAKA R, CHENAL C, MISSET M T. Effects of low doses of short-term gamma irradiation on growth and development through two generations of Pisum sativum.[J]. The Science of the total environment, 2004, 320(2-3).
[31]	李丽辉, 胡瑶, 雷星宇, 等. ~(60)Co-γ射线辐射对4种百合的诱变效应[J]. 核农学报, 2021, 35(8):1725-1730.
[32]	张玉, 白史且, 李达旭, 等. ~(60)Co-γ辐射对莴苣种子发芽及幼苗生理的影响[J]. 草地学报, 2013, 21(1):147-151.
[33]	李波, 刘畅, 李红, 等. ~(60)Co-γ辐射苜蓿种子的变异植株叶片愈伤组织诱导及其诱变效应分析[J]. 干旱地区农业研究, 2021(2):164-171.
[34]	朱宏芬, 刘健, 黄坚. ~(60)Co-γ射线对蓝莓组培苗的诱变效应[J]. 黑龙江农业科学, 2018(5):21-25.
[35]	廖安红, 陈红. 不同~(60)Co-γ射线辐照剂量对刺梨幼苗生长及生理特性的影响[J]. 分子植物育种, 2016, 14(3):742-748.
[36]	吴建慧, 牛喆, 兰凤, 等. ~(60)Co-γ射线辐射对2种露地菊叶片生理生化特性的影响[J]. 核农学报, 2019, 33(6):1059-1064.

辐射剂量/Gy	发芽率/%	成苗率/%	发芽势/%
0	69.67±3.51Aa	68.33±4.04Aa	60.00±3.46Aa
5	68.00±3.46Aa	59.00±1.00Bb	37.00±4.58Bb
10	49.00±2.65Bb	42.67±2.52Cc	26.67±4.93Cc
20	12.33±2.08Cc	10.00±1.73Dd	6.67±1.15Dd
30	4.33±1.53Dd	2.33±1.53Ee	0.67±1.15Ed
40	0.33±0.58Dd	0.00±0.00Ee	0.00±0.00Ed

辐射剂量/Gy	发芽率/%	成苗率/%	发芽势/%
0	69.67±3.51Aa	68.33±4.04Aa	60.00±3.46Aa
5	68.00±3.46Aa	59.00±1.00Bb	37.00±4.58Bb
10	49.00±2.65Bb	42.67±2.52Cc	26.67±4.93Cc
20	12.33±2.08Cc	10.00±1.73Dd	6.67±1.15Dd
30	4.33±1.53Dd	2.33±1.53Ee	0.67±1.15Ed
40	0.33±0.58Dd	0.00±0.00Ee	0.00±0.00Ed

剂量/Gy	90 d		120 d		150 d
剂量/Gy	增殖率/%	苗高/cm	增殖率/%	苗高/cm	增殖率/%	苗高/cm
0	159.67±16.01Aa	2.38±0.09Aa	273.00±7.55Aa	2.72±0.15Aa	297.67±8.74Aa	3.04±0.07Aa
5	137.33±11.01Ba	1.89±0.11Bb	254.67±5.51Ba	2.07±0.06Bb	185.00±7.55Aa	2.41±0.10Bb
10	96.67±11.24Cb	1.71±0.06Cb	159.67±5.69Cb	2.03±0.16Bb	188.33±15.01Bb	2.27±0.11BCb
20	44.00±6.56Dc	1.47±0.06Dc	62.33±11.93Dc	1.71±0.05Cc	130.67±46.97Cc	2.12±0.18Cb
30	24.00±6.25Ecd	1.37±0.06Dc	40.67±13.58Ecd	1.59±0.08Cc	98.00±9.85Cc	1.79±0.09Dc
40	9.33±3.51Ed	1.01±0.09Ed	34.00±5.57Ed	1.22±0.13Dd	42.00±13.00Dd	1.48±0.12Ed

剂量/Gy	90 d		120 d		150 d
剂量/Gy	增殖率/%	苗高/cm	增殖率/%	苗高/cm	增殖率/%	苗高/cm
0	159.67±16.01Aa	2.38±0.09Aa	273.00±7.55Aa	2.72±0.15Aa	297.67±8.74Aa	3.04±0.07Aa
5	137.33±11.01Ba	1.89±0.11Bb	254.67±5.51Ba	2.07±0.06Bb	185.00±7.55Aa	2.41±0.10Bb
10	96.67±11.24Cb	1.71±0.06Cb	159.67±5.69Cb	2.03±0.16Bb	188.33±15.01Bb	2.27±0.11BCb
20	44.00±6.56Dc	1.47±0.06Dc	62.33±11.93Dc	1.71±0.05Cc	130.67±46.97Cc	2.12±0.18Cb
30	24.00±6.25Ecd	1.37±0.06Dc	40.67±13.58Ecd	1.59±0.08Cc	98.00±9.85Cc	1.79±0.09Dc
40	9.33±3.51Ed	1.01±0.09Ed	34.00±5.57Ed	1.22±0.13Dd	42.00±13.00Dd	1.48±0.12Ed

剂量/Gy	120 d				150 d
剂量/Gy	生根率/%	生根数/条	平均根长/cm	根系活力	生根率/%	生根数/条
0	53.67±4.16Aa	112. 67±6.51Aa	0.34±0.04Aa	34.34±2.43Aa	82.67±6.03Aa	252.00±11.14Aa
5	37.33±2.52Bb	73.33±4.51Bb	0.22±0.01Bb	33.50±2.17Aa	71.67±3.06Bb	180.33±3.51Bb
10	16.33±1.53Cc	20.33±2.52Cc	0.14±0.00Cbc	22.74±2.76Bb	21.00±1.73Cc	44.00±4.58Cc
20	5.33±1.15Dd	6.33±1.15Dd	0.09±0.07Cc	11.46±0.53Cc	6.33±0.58Dd	9.33±0.58Dd
30	0Ed	0Dd	0Dd	0Dd	0Ed	0Dd
40	0Ed	0Dd	0Dd	0Dd	0Ed	0Dd
剂量/Gy	150 d		180 d
剂量/Gy	平均根长/cm	根系活力	生根率/%	生根数/条	平均根长/cm	根系活力
0	1.37±0.08Aa	38.38±2.34Aa	94.67±2.31Aa	270.00±8.89Aa	2.15±0.09Aa	39.27±1.79Aa
5	1.16±0.08Bb	38.30±1.46Aa	73.00±4.58Bb	194. 67±9.29Bb	2.06±0.07Aa	40.86±0.85Aa
10	0.62±0.07Cc	22.87±1.88Bb	21.33±2.08Cc	44.00±3.61Cc	1.40±0.06Bb	26.34±1.41Bb
20	0.37±0.04Dd	13.55±0.70Cc	6.67±0.58Dd	8.33±1.15Dd	0.60±0.07Cc	15.37±0.92Cc
30	0Ee	0Dd	0.33±0.58Ee	0.33±0.58Dd	0.08±0.13Dd	7.71±1.30Dd
40	0Ee	0Dd	0Ee	0Dd	0Dd	0Ee

Mutagenic Effect of ⁶⁰Co-γ on Tissue Culture Seedlings of Paphiopedilum

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 11

References 36

Related Articles 15

Recommended Articles

Metrics

Comments

For authors

For reviewers

Reader center

Contact us

剂量/Gy	株高生长量/cm	根系生长量/cm	生长情况	变异情况
0	2.92±0.14Aa	2.14±0.11Aa	生长整齐,长势旺盛,叶片鲜绿	无变异
5	2.82±0.12ABa	2.11±0.14Aab	生长整齐、长势较旺盛,叶片鲜绿	未见明显变异
10	2.67±0.12Ba	1.90±0.07Bbc	生长不整齐,少数植株长势较弱、株型低矮	极少数叶片变细长
20	2.35±0.16Cb	1.73±0.03Cc	生长不整齐,部分植株长势弱、低矮, 少数叶缘有焦边和褐化现象	叶形出现卷曲、缺裂、细长等变异、叶色变浅
30	1.96±0.09Dc	1.73±0.08Cc	生长不整齐,部分植株长势弱、低矮, 叶缘有焦边和褐化现象	叶形出现卷曲、缺裂、细长等变异比例较大、出现花叶,黄化叶
40	0.93±0.09Ed	1.42±0.08Dd	生长不整齐,长势极弱, 叶缘褐化现象严重	叶片卷曲和缺裂等变异严重, 叶片褐化面积大

辐射剂量/Gy	可溶性糖/（mg/g鲜重）	可溶性蛋白/（mg/g鲜重）	脯氨酸Pro/（μg/g鲜重）
0	24.32±2.01Ed	7.57±0.86CDc	25.66±3.11Dc
5	45.48±4.06Dc	9.12±0.96Cc	37.59±2.21Cb
10	59.53±4.01Bb	14.92±1.43Bab	46.50±3.40Bb
20	70.18±1.55Aa	17.37±1.19Aa	66.05±3.54Aa
30	51.41±4.18Cbc	13.75±1.55Bb	71.59±3.12Aa
40	27.03±3.04Ed	6.96±0.37Dc	41.98±4.93BCb

辐射剂量/Gy	SOD/（U/g鲜重)	POD/（U/g鲜重）
0	54.30±3.09Cc	423.25±11.79Cc
5	103.65±4.00Bb	482.41±24.60Bb
10	157.47±5.15Aa	614.46±5.95Aa
20	157.15±3.83Aa	509.90±20.53Bb
30	110.16±7.23Bb	488.10±14.37Bb
40	52.67±3.07Cc	368.84±15.01Dd

[1]	WANG Sizhi, GUAN Wenling, HAO Xiaohan, SONG Jie. Study on Germination Characteristics of Gaultheria procumbens Seeds [J]. Chinese Agricultural Science Bulletin, 2023, 39(1): 77-84.
[2]	SHEN Jicheng, ZHAO Caixia, YE Fahui, LI Yaxin, LIU Demei, LIU Ruijuan, SHEN Yuhu, ZHANG Huaigang, CHEN Wenjie. Analysis of Germplasm Differentiation of Ledu Long Pepper Based on Agronomic Traits [J]. Chinese Agricultural Science Bulletin, 2022, 38(7): 29-34.
[3]	XU Xinyang, ZHANG Yuejian, SHEN Jia, SHOU Weisong. Methods for Breaking Seed Dormancy of Thick-skinned Muskmelon ‘Cuixue 5’ [J]. Chinese Agricultural Science Bulletin, 2022, 38(7): 41-44.
[4]	LI Lu, BAO Xinyi, CHEN Zhiyong. Study on Tissue Culture Technique of Saintpantia ionantha [J]. Chinese Agricultural Science Bulletin, 2022, 38(6): 58-62.
[5]	BAI Yanrong, JIANG Yalian, WANG Jinying. Study on Tissue Culture and Rapid Propagation System of Chamelaucium uncinatum [J]. Chinese Agricultural Science Bulletin, 2022, 38(16): 46-50.
[6]	YANG Wenjing, WANG Zhanjun, GAN Xiaoyan, HE Jianlong, ZHANG Li. The Establishment of Efficient Regeneration System of Cerasus humilis [J]. Chinese Agricultural Science Bulletin, 2022, 38(13): 60-65.
[7]	JIN Wu, MA Xueyan, LV Guohua, GU Ruobo, HUA Dan, WEN Haibo. A Review of Reproductive Biology of Gastropoda [J]. Chinese Agricultural Science Bulletin, 2022, 38(11): 116-122.
[8]	WANG Yinhua, REN Fei, LI Qinghua, ZHAI Guofeng, ZANG Zhenrong, WU Dejun, YAN Liping, YAO Junxiu. Study on Softwood Cutting Propagation Technology of Sambucus williamsii [J]. Chinese Agricultural Science Bulletin, 2022, 38(10): 26-31.
[9]	SHI Jiaqi, SA Rula, LING Yu, SA Rulaqiqige, FAN Fu, ZHANG Xueting. Effects of Straw Addition in Saline-alkaline Soil on Seed Germination and Seedling Growth Characteristics of Maize [J]. Chinese Agricultural Science Bulletin, 2021, 37(36): 13-18.
[10]	Li Wenming, Ma Jiancang, Lei Yuming, Xing Huiqin, Shang Liqi. Effect of Different Temperature and Time on Seed-borne Fungi and Seed Germination of Maize [J]. Chinese Agricultural Science Bulletin, 2021, 37(32): 10-14.
[11]	Fang Wei, Huang Heliang. Calculation and Analysis of Spatial Evolution and Barycenter Change of Grain Production Based on County Units: Taking Guangdong Province as an Example [J]. Chinese Agricultural Science Bulletin, 2021, 37(3): 150-159.
[12]	He Yongheng, Feng Guojun, Liu Dajun, Liu Chang, Yang Xiaoxu, Yan Zhishan. Study on the Growth, Development and Physiological Characteristics of Common Beans [J]. Chinese Agricultural Science Bulletin, 2021, 37(28): 43-49.
[13]	Lan Jinxuan, Liang Wenhui, Huang Xiaolu, Yang Zhuoying, Li Baocai, Ma Jinlin. Anatomical Observation on Cutting Propagation and Adventitious Root Formation of Vernicia fordii [J]. Chinese Agricultural Science Bulletin, 2021, 37(19): 42-46.
[14]	Sa Rula, Zhang Xueting, Yang Hengshan, Zhang Yun, Xu Yonghui. Straw Fermentation Broth: Effects on Seed Germination and Seedling Growth Characteristics of Maize [J]. Chinese Agricultural Science Bulletin, 2021, 37(18): 14-18.
[15]	Wang Jinghong, Chen Ai, Zhang Wen, Zhao Yi, Tian Jingyao, Wang Zhensheng, Xu Gudan, Lin Jixiang. Rooting Agent (GGR-6): Effects on the Response of Festuca rubra and Poa pratensis to Drought Stress [J]. Chinese Agricultural Science Bulletin, 2021, 37(15): 47-54.