Abstract:

To find out the best medium for inducing the first generation and multiplying the second generation and rooting, the tissue culture technique system of Chamelaucium uncinatum was established. The tender shoot tip of C. uncinatum was used as the experimental material, MS medium was used to add KT and NAA with different proportions, the first generation of C. uncinatum was induced, different concentrations of 6-BA and NAA were combined for proliferation, the 1/2MS medium was selected as the basic medium for the rooting culture of C. uncinatum, and different concentrations of IBA were added to the medium. The best medium for bud induction was MS+ KT 3.0 mg/L+ NAA 0.2 mg/L+ agar 6.2 g/L+ sucrose 30 g/L with pH 5.8, and the induction rate was 88.8%. The best medium for proliferation was MS+ 6-BA 1.0 mg/L+ NAA 0.1 mg/L+ agar 6.2 g/L+ sucrose 30 g/L with pH 5.8, and the proliferation coefficient was 3.6. The best rooting medium was 1/2MS+ IBA 0.6 mg/L+ agar 6.2 g/L+ sucrose 30 g/L+ 0.1 g/L activated carbon with pH 5.8, and the rooting rate was 100%.

Key words: Chamelaucium uncinatum, induction culture, proliferation culture, rooting culture, culture medium