Abstract: The research made a serial of studies on buds inducement and reproduction, root differentiation and cultivation of young plant in different hormone and different culture media, which selected tender leaves of Pelargonium citrosum as explants in germfree condition. The results showed that MS+6-BA1.0mg/L+NAA0.1mg/L was good for buds inducement and the rate of inducement is 96%, that MS（B5）+6-BA0.5mg/L +NAA0.1mg/L are suitable for buds reproduction, the coefficient of reproduction is up to 8, and buds grew very well, that MS（B5）+6-BA0.1mg/L+NAA0.2mg/L were good for getting stronger, and that 1/2MS+NAA0.1 mg/L and MS+IBA0.2 mg/L were best for rootage, the rooting rate is 100% , and the young plants grew very well. The main factors affecting its large-scale production are concentration of hormone, times of subculture and transplant manage.