Effects of Rain Shelter Cultivation on Growth, Hormones and Yield of Line Pepper in Sichuan Basin

doi:10.11924/j.issn.1000-6850.casb2023-0798

Abstract

Abstract:

To investigate the effects of sheltered rain-avoiding cultivation on the growth, hormones, and yield of chili pepper ‘Sichuan 20’ in the Sichuan basin, and clarify the influencing mechanism, we set up sheltered rain-avoiding cultivation treatment and field control treatment, measured the height of chili pepper plants and the content of endogenous hormones in the apical tissues of chili pepper plants in the two treatments, and conducted transcriptome sequencing and qRT-PCR validation of related genes. The results showed that there was no significant difference in chili pepper plant height in the early period of sheltered rain-avoiding cultivation, and the plant height in the middle and late periods of sheltered rain-avoiding cultivation was 1.13 and 1.5 times that of the field control treatment, respectively. The yield per plant in the sheltered rain-avoiding cultivation treatment was 74.9% of that in the field control treatment. After sheltered rain-avoiding cultivation, the content of gibberellin was 1.29 times that of the field control treatment, and the content of jasmonic acid was 63.3% of that of the field control treatment. GO analysis showed that the two treatments were significantly enriched in biological functions related to stress response and defense response and were significantly enriched in molecular functions related to adenosine nucleotide binding, adenosine diphosphate binding, and guanosine diphosphate binding. KEGG pathway analysis showed that compared with the sheltered rain-avoiding cultivation treatment, the field control treatment of chili pepper in the sesquiterpene biosynthesis metabolic pathway had four genes related to gibberellin degradation, CaGA2ox1.1(Gibberellin 2-beta-dioxygenase 1, T459_20483), CaGA2ox1.2 (Gibberellin 2-beta-dioxygenase 1, T459_24753), CaGA2ox8(Gibberellin 2-beta-dioxygenase 8, T459_05864), CaGRP3(Gibberellin-regulated protein 3, T459_06266). After the installation of facilities for rain protection cultivation in the Sichuan basin, the expression level of the gene for degradation of gibberellin at the top of the chili pepper decreased, while the content of gibberellin increased, causing the excessive growth of chili pepper, ultimately leading to a lower yield of chili pepper in the facilities for rain protection cultivation than in the field cultivation.

Key words: line pepper, rain shelter cultivation, hormones, yield

ZHANG Zejin, LIANG Ying, TANG Li. Effects of Rain Shelter Cultivation on Growth, Hormones and Yield of Line Pepper in Sichuan Basin[J]. Chinese Agricultural Science Bulletin, 2024, 40(25): 59-65.

Figures/Tables 6

References 26

[1]	刘娟, 吴传秀, 李艳红, 等. 推动川椒产业高质量发展的对策建议[J]. 四川农业科技, 2020(11):68-70.
[2]	张泽锦, 梁颖, 王力明, 等. 基于温度和降雨量分析四川茄果类蔬菜周年生产布局[J]. 四川农业科技, 2022(8):24-27.
[3]	付一峰, 唐丽, 张泽锦. 四川盆地番茄不同栽培密度对产量和品质的影响[J]. 中国瓜菜, 2022, 35(7):75-78.
[4]	ACHARD P, GENSCHIK P. Releasing the brakes of plant growth: How GAs shutdown DELLA proteins[J]. Journal of experimental botany, 2017, 60(4):1085-1092.
[5]	梁任繁, 苏义成, 仇惠君, 等. 叶面喷施多效唑对网纹甜瓜幼苗农艺性状、内源激素的影响及其相互关系[J]. 中国瓜菜, 2023, 36(6):50-57.
[6]	桑嘉骏, 季延海, 刘明池, 等. 矮壮素施用方式和灌溉水温对黄瓜幼苗徒长的调控研究[J]. 中国蔬菜, 2022(6):50-55.
[7]	韩佳宇, 林玲, 曹雄军, 等. 摘心与矮壮素处理对“夏黑”葡萄枝条内源激素及生长的影响[J]. 中国南方果树, 2021, 50(5):116-118.
[8]	卢环, 王成, 曾玲玲, 等. 不同浓度烯效唑浸种对绿豆生理及生长的影响[J]. 黑龙江农业科学, 2023(8):50-57.
[9]	张泽锦, 王力明, 梁颖, 等. 喷施矮壮素和烯效唑对四川盆地设施线椒生长和产量的影响[J]. 四川农业科技, 2022(7):31-33.
[10]	王雪艳, 姜艺昊, 朱静如, 等. 抑制辣椒幼苗植物生长延缓剂对辣椒生长发育的影响[J]. 浙江农业科学, 2021, 62(5):873-877. doi: 10.16178/j.issn.0528-9017.20210509
[11]	曹振木, 牛玉, 刘子记, 等. 矮壮素及烯效唑对甜椒幼苗质量的影响[J]. 热带作物学报, 2012, 33(12):2156-2160.
[12]	唐丽, 王海娥, 李小玲, 等. 避雨栽培对番茄生长微环境及病害发生的影响[J]. 西南农业学报, 2012, 25(6):2021-2025.
[13]	付一峰, 张泽锦, 唐丽. 避雨栽培对四川盆地春季茄子光合电子传递、产量及品质的影响[J]. 中国瓜菜, 2023, 36(8):12-18.
[14]	胡晨曦, 李子恒, 张云虹, 等. 低温弱光对不同品种辣椒幼苗生长和光合特性的影响[J]. 福建农业学报, 2022, 37(5):617-625.
[15]	MAO L Z, DAI Y H, HUANG Y, et al. Effect of light intensity on gene expression in hypocotyl during the elongation in a leaf-yellowing mutant of pepper (Capsicum annuum L.)[J]. Agronomy, 2022, 12(11):2762-2762.
[16]	李欣, 李影, 曲子越, 等. bHLH转录因子在茉莉酸信号诱导植物次生产物合成中的作用及分子机制[J]. 植物生理学报, 2017, 53(1):1-8.
[17]	NIEUWENHUIZEN N J, CHEN X Y, WANG M Y, et al. Natural variation in monoterpene synthesis in kiwifruit: transcriptional regulation of terpene synthases by NAC and ETHYLENE-INSENSITIVE3-Like transcription factors[J]. Plant physiology, 2015, 167(4):1243-1258. doi: 10.1104/pp.114.254367 pmid: 25649633
[18]	张凯伦, 罗祖良, 郭玉华, 等. bHLH转录因子调控药用植物萜类化合物生物合成的研究进展[J]. 中国现代中药, 2017, 19(1):142-147.
[19]	YAMAMURA C, MIZUTANI E, OKADA K, et al. Diterpenoid phytoalexin factor, a bHLH transcription factor, plays a central role in the biosynthesis of diterpenoid phytoalexins in rice[J]. The plant journal, 2015, 84(6):1100-1113. doi: 10.1111/tpj.13065 pmid: 26506081
[20]	SHANG Y, MA Y S, ZHOU Y, et al. Biosynthesis, regulation, and domestication of bitterness in cucumber[J]. Science, 2014, 346:1084-1088.
[21]	LV Z Y, WANG S, ZHANG F Y, et al. Overexpression of a novel NAC domain-containing transcription factor gene (AaNAC1) enhances the content of artemisinin and increases tolerance to drought and Botrytis cinerea in Artemisia annua[J]. Plant and cell physiolog, 2016, 57(9):1961-1971.
[22]	PHILLIPS A L, UKNES S, APPLEFORD N E J, et al. Isolation and expression of three gibberellin 20-oxidase cDNA clones from Arabidopsis[J]. Plant physiology, 1995, 108(3):1049-1057.
[23]	冯怡. 棉花GA20-氧化酶基因GhGA20ox1超量表达及其对番茄果实发育的影响[D]. 重庆: 西南农业大学, 2005.
[24]	王江英, 吴斌, 刘伟鑫, 等. 矮生山茶‘恨天高’GA20ox1的克隆及其对转基因烟草株形的影响[J]. 园艺学报, 2015, 42(8):1523-1532. doi: 10.16420/j.issn.0513-353x.2014-1045
[25]	王月华. 结缕草GA20氧化酶基因的克隆及遗传转化研究[D]. 北京: 北京林业大学, 2007.
[26]	张熠. 荫蔽下赤霉素调节大豆茎秆抗倒的生理机制[D]. 雅安: 四川农业大学, 2022.

处理	赤霉素/(pmol/g)	细胞分裂素/(ng/g)	生长素/(nmol/g)	脱落酸/(ng/g)	茉莉酸/(pmol/g)
避雨栽培	105.75±7.51a	40.67±6.48a	31.11±7.78a	58.93±11.96a	9.85±2.54b
露地栽培	82.19±9.36b	43.20±9.05a	27.85±8.23a	59.22±13.38a	15.56±2.41a

处理	赤霉素/(pmol/g)	细胞分裂素/(ng/g)	生长素/(nmol/g)	脱落酸/(ng/g)	茉莉酸/(pmol/g)
避雨栽培	105.75±7.51a	40.67±6.48a	31.11±7.78a	58.93±11.96a	9.85±2.54b
露地栽培	82.19±9.36b	43.20±9.05a	27.85±8.23a	59.22±13.38a	15.56±2.41a