Synergistic Mechanism of Application of Trichoderma harzianum and Amendments on Physicochemical Properties of Saline-alkali Soil and Cotton’s Salt Alkali Resistance Response

doi:10.11924/j.issn.1000-6850.casb2025-0271

Abstract

Abstract:

This study investigated the synergistic ameliorative effects of Trichoderma harzianum in combination with different soil amendments on saline-alkali soils, and their impact on cotton growth. A pot experiment was conducted with six treatments: control (CK), T. harzianum alone (T), T. harzianum combined with biochar (BT), humic acid (HT), organic fertilizer (AT), and microbial inoculant (MT). The effects on soil physicochemical properties and the physiological performance of cotton were comprehensively assessed. Results showed that the AT treatment (combination with organic fertilizer) reduced soil pH by 2.58%, significantly outperforming other treatments. It also markedly increased the contents of soil organic matter, available phosphorus, available potassium, and alkali-hydrolyzable nitrogen, indicating that the amendments significantly improved soil physicochemical conditions. Regarding plant growth parameters, the AT treatment significantly enhanced plant height (+36.40%), stem diameter (+49.50%), aboveground biomass (+68.67%), and belowground biomass (+89.29%), representing the highest increases among all treatments. Physiological and biochemical analyses revealed that T, AT, and MT treatments significantly increased the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in cotton leaves, while reducing the levels of malondialdehyde (MDA), soluble protein, and proline, indicating enhanced stress resistance. Correlation analysis further demonstrated significant relationships between soil properties and cotton growth indicators, providing a scientific basis for optimizing soil amendment strategies. In conclusion, the combination of T. harzianum and organic fertilizer (AT treatment) was most effective in improving soil quality and promoting cotton growth. These findings offer scientific support and practical guidance for the efficient utilization of saline-alkali soils and the sustainable production of high-quality cotton in Xinjiang.

Key words: Trichoderma harzianum, amendment, soil physical and chemical properties, cotton, growth and physiological indicators

WAN Tingting, HE Hao, CHENG Liyang, LI Shuai, YU Mengmeng, QIN Jie, LI Shikuo, LI Junhua. Synergistic Mechanism of Application of Trichoderma harzianum and Amendments on Physicochemical Properties of Saline-alkali Soil and Cotton’s Salt Alkali Resistance Response[J]. Chinese Agricultural Science Bulletin, 2025, 41(21): 145-155.

Figures/Tables 7

References 42

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处理	株高/cm	茎粗/mm	地上部生物量/(g/pot)	地下部生物量/(g/pot)
CK	11.21±0.49c	3.03±0.08d	0.83±0.01c	0.28±0.01cd
T	13.20±0.15b	3.55±0.10c	0.90±0.01bc	0.33±0.02c
BT	13.80±0.12b	3.65±0.11bc	0.92±0.05b	0.30±0.03cd
HT	10.46±1.06c	3.06±0.07d	0.71±0.02d	0.25±0.01d
AT	15.29±0.72a	4.53±0.36a	1.40±0.04a	0.53±0.04a
MT	13.83±0.51b	3.95±0.04b	0.97±0.04b	0.41±0.04b

处理	株高/cm	茎粗/mm	地上部生物量/(g/pot)	地下部生物量/(g/pot)
CK	11.21±0.49c	3.03±0.08d	0.83±0.01c	0.28±0.01cd
T	13.20±0.15b	3.55±0.10c	0.90±0.01bc	0.33±0.02c
BT	13.80±0.12b	3.65±0.11bc	0.92±0.05b	0.30±0.03cd
HT	10.46±1.06c	3.06±0.07d	0.71±0.02d	0.25±0.01d
AT	15.29±0.72a	4.53±0.36a	1.40±0.04a	0.53±0.04a
MT	13.83±0.51b	3.95±0.04b	0.97±0.04b	0.41±0.04b

处理	叶绿素a含量	叶绿素b含量	类胡萝卜素含量	叶绿素总量
CK	3.833±0.086d	2.203±0.156c	0.637±0.028c	6.037±0.137d
T	4.430±0.268cd	2.596±0.139bc	0.838±0.031b	7.027±0.155cd
BT	4.831±0.161c	2.564±0.219bc	0.839±0.019b	7.395±0.058c
HT	5.851±0.173b	3.104±0.207ab	0.928±0.078ab	8.954±0.142b
AT	7.016±0.184a	3.389±0.193a	1.053±0.115a	10.406±0.177a
MT	4.938±0.130c	2.470±0.100bc	0.813±0.017b	7.408±0.227c

处理	叶绿素a含量	叶绿素b含量	类胡萝卜素含量	叶绿素总量
CK	3.833±0.086d	2.203±0.156c	0.637±0.028c	6.037±0.137d
T	4.430±0.268cd	2.596±0.139bc	0.838±0.031b	7.027±0.155cd
BT	4.831±0.161c	2.564±0.219bc	0.839±0.019b	7.395±0.058c
HT	5.851±0.173b	3.104±0.207ab	0.928±0.078ab	8.954±0.142b
AT	7.016±0.184a	3.389±0.193a	1.053±0.115a	10.406±0.177a
MT	4.938±0.130c	2.470±0.100bc	0.813±0.017b	7.408±0.227c