Abstract: The in-vitro plate culture and in-vivo inoculation tests were conducted to study biological activities of bleeding sap of sponge gourd on pepper Botrytis cinerea, tomato Alternaria Solani and apple Glomerella cingulata. The results showed that bleeding sap had quite strong fungistatic activities on plant pathogenic fungi. The in-vitro plate culture indicated that the fungistatic rates of 0.3 volume fraction of HD bleeding sap(at the rate of medium ) on three plant pathogenic fungi were 90.70%, 36.97%，40.87%, respectively. The in-vivo inoculation tests indicated that the prevention rates of 0.5 volume fraction of HD bleeding sap on pepper Botrytis cinerea, tomato Alternaria Solani, and apple Glomerella cingulata were 73.60%, 47.12%, and 42.84%, respectively. The bleeding sap had the best fungistatic effect on Botrytis cinerea among the three pathogenic fungi.