Abstract: Dynamic the effective function of biological seed coating ZSB and changes of in active of peroxideases (POD), polyphenoloxides (PPO) and phenylalantine ammonia lyases (PAL) in two resistant cultivars and two susceptible ones of maize after inoculating Rhizoctonia solani. ZSB was assured with effective function to control sheath blight of four cultivars of maize, and the effective function to Washi13 and Shenshi28 was 39% and 49.7%, higher than to Yedan13 and Shenshi29. It was more likely that ZSB control effect was devoted by its induction to PPO and POD in host prior to and higher than inoculation with R. solani,. It should be noted that some special phenomenon needed to be further investigated such as still no significant increase of PAL.