Abstract: Interspecific hybrid of Brassica napus and Brassica oleracea L. var. italica Phenck was obtained by in vitro tissue culture of ovaries. The best data of in vitro culture of ovaries, which was pollinated 15 days ago, resulted in 7 full seeds (including 2 sprouts) and a germination rate of 29%. The most appropriate culture medium was 1/2MS+B5 organic + IAA 1.5mg/L + sucrose 50g/L on which we had average 0.17 hybrid seeds per legume and a germination rate of 66.7%. We drew a conclusion that biological characters of this interspecific hybrid were mostly similar to those of male parent in comparison with both parents.