Abstract: Investigations were carried out to evaluate the influence of low Zn tolerant on plant growth, antioxidant enzymes, root system vigor, chlorophyll and absorption and utilization of Zn in three varieties of rape (Brassica napus L.) by solution experiment. The results showed that significant differences of tolerant were recorded in response to low Zn2+ level (10-6mol/L Zn2+) among the rape seedlings due to different genotypes. Under low Zn treatments, shoot dry weight of seedlings was in the order of Brassica juncea> Brassica napus > Brassica campestris, and increased by 17.8% ( Brassica junce), 9.9% (Brassica campestri), 3.3% ( Brassica napus) compared to the control. The higher activities of CAT, POD, SOD, concentration of chlorophyll, and root tolerance were found in Brassica juncea, and its Zn uptake and Zn utilization efficiency were higher than Brassica napus and Brassica campestris. Zn utilization efficiency of the three varieties of rape was 12.3%（Brassica junce）, 8.0% ( Brassica campestri), 4.9% (Brassica napus), respectively. Antioxidant enzyme activity, content of chlorophyll, root system vigor of rape seedlings could be act as the biomarkers for screening rape genotypes tolerant to low Zn.